Publicação
Modelling and optimization of ethyl butyrate production catalysed by Rhizopus oryzae lipase
| Resumo: | Response surface methodology was used to model and optimise the production of ethyl butyrate, catalysed by Rhizopus oryzae lipase immobilised in a hydrophilic polyurethane foam. Experiments were carried out following a central composite rotatable design, as a function of reaction temperature (T: 22–38 C) initial butyric acid concentration (A: 0.031–0.619 M) and initial molar ratio ethanol/acid (MR; 0.257–2.443). After 48 h reaction time, the production of ethyl butyrate could be fitted to a surface described by a second-order polynomial model. A maximum ethyl butyrate concentration of 0.106 M, corresponding to 47% conversion into ester and a productivity of 2.21 lmole/mL h, is expected at initial reaction conditions of T, A and MR of 33 C, 0.225 M and 1.637, respectively. This maximum was experimentally confirmed |
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| Autores principais: | Grosso, C. |
| Outros Autores: | Ferreira-Dias, Suzana; Pires-Cabral, P. |
| Assunto: | ethyl butyrate immobilised lipase Rhizopus oryzae modelling optimization polyurethane foam |
| Ano: | 2013 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório da Universidade de Lisboa |
| Resumo: | Response surface methodology was used to model and optimise the production of ethyl butyrate, catalysed by Rhizopus oryzae lipase immobilised in a hydrophilic polyurethane foam. Experiments were carried out following a central composite rotatable design, as a function of reaction temperature (T: 22–38 C) initial butyric acid concentration (A: 0.031–0.619 M) and initial molar ratio ethanol/acid (MR; 0.257–2.443). After 48 h reaction time, the production of ethyl butyrate could be fitted to a surface described by a second-order polynomial model. A maximum ethyl butyrate concentration of 0.106 M, corresponding to 47% conversion into ester and a productivity of 2.21 lmole/mL h, is expected at initial reaction conditions of T, A and MR of 33 C, 0.225 M and 1.637, respectively. This maximum was experimentally confirmed |
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