Publicação
A Simple Double-Spin Closed Method for Preparing Platelet-Rich Plasma
| Resumo: | Objective: To describe and analyze a new protocol for the extraction of platelet-rich plasma (PRP) for use in clinical practice and compare this technique with methods that have been previously described in the medical literature. Methods: Sixteen blood samples from healthy volunteers were collected. PRP was prepared using our new double-spin technique, consisting of successive centrifugation of blood samples with two different spins, without opening the container. Descriptive analysis of cell counts in baseline and PRP samples was undertaken. Comparison between cell and platelet count in baseline and PRP samples, as well as the statistical analysis, were done. Results: The mean platelet concentration ratio was 3.47 (SD: 0.85; 95% CI: 3.01-3.92; range: 2.48-5.71). The baseline whole blood platelet count correlated positively to the PRP platelet count (rP = 0.56; 95% CI: 0.09- 0.88; P = 0.023). The PRP was enriched for lymphocytes and monocytes but presented significantly lower counts of neutrophils and eosinophils in comparison to baseline. Conclusion: Results show a safe and easily reproducible method to obtain PRP for use in clinical daily practice. |
|---|---|
| Autores principais: | Machado, Edilson S |
| Outros Autores: | Soares, Fabiano P; Yamaguchi, Roberta S; Felipone, William K; Meves, Robert; Souza, Tais Amara C; Topolniak, Roberto; Caldas, José P; Abreu, Ernani V; Rabelo Neto, Luiz S; Pinchemel, Pedro Vinicius S; Bredemeier, Markus |
| Assunto: | platelets, double-spin, growth factors, orthobiologics, orthopedics, regenerative medicine, platelet-rich plasma Pain Management, Physical Medicine & Rehabilitation, Orthopedic |
| Ano: | 2022 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório da Universidade de Lisboa |
| _version_ | 1866810384871063552 |
|---|---|
| author | Machado, Edilson S |
| author2 | Soares, Fabiano P Yamaguchi, Roberta S Felipone, William K Meves, Robert Souza, Tais Amara C Topolniak, Roberto Caldas, José P Abreu, Ernani V Rabelo Neto, Luiz S Pinchemel, Pedro Vinicius S Bredemeier, Markus |
| author2_role | author author author author author author author author author author author |
| author_facet | Machado, Edilson S Soares, Fabiano P Yamaguchi, Roberta S Felipone, William K Meves, Robert Souza, Tais Amara C Topolniak, Roberto Caldas, José P Abreu, Ernani V Rabelo Neto, Luiz S Pinchemel, Pedro Vinicius S Bredemeier, Markus |
| author_role | author |
| contributor_name_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Machado, Edilson S\"},{\"Person.name\":\"Soares, Fabiano P\"},{\"Person.name\":\"Yamaguchi, Roberta S\"},{\"Person.name\":\"Felipone, William K\"},{\"Person.name\":\"Meves, Robert\"},{\"Person.name\":\"Souza, Tais Amara C\"},{\"Person.name\":\"Topolniak, Roberto\"},{\"Person.name\":\"Caldas, José P\"},{\"Person.name\":\"Abreu, Ernani V\"},{\"Person.name\":\"Rabelo Neto, Luiz S\"},{\"Person.name\":\"Pinchemel, Pedro Vinicius S\"},{\"Person.name\":\"Bredemeier, Markus\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| datacite.creators.creator.creatorName.fl_str_mv | Machado, Edilson S Soares, Fabiano P Yamaguchi, Roberta S Felipone, William K Meves, Robert Souza, Tais Amara C Topolniak, Roberto Caldas, José P Abreu, Ernani V Rabelo Neto, Luiz S Pinchemel, Pedro Vinicius S Bredemeier, Markus |
| datacite.date.Accepted.fl_str_mv | 2022-01-03T00:00:00Z |
| datacite.date.available.fl_str_mv | 2023-09-19T15:25:15Z |
| datacite.date.embargoed.fl_str_mv | 2023-09-19T15:25:15Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | platelets, double-spin, growth factors, orthobiologics, orthopedics, regenerative medicine, platelet-rich plasma Pain Management, Physical Medicine & Rehabilitation, Orthopedic |
| datacite.titles.title.fl_str_mv | A Simple Double-Spin Closed Method for Preparing Platelet-Rich Plasma |
| dc.contributor.none.fl_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| dc.creator.none.fl_str_mv | Machado, Edilson S Soares, Fabiano P Yamaguchi, Roberta S Felipone, William K Meves, Robert Souza, Tais Amara C Topolniak, Roberto Caldas, José P Abreu, Ernani V Rabelo Neto, Luiz S Pinchemel, Pedro Vinicius S Bredemeier, Markus |
| dc.date.Accepted.fl_str_mv | 2022-01-03T00:00:00Z |
| dc.date.available.fl_str_mv | 2023-09-19T15:25:15Z |
| dc.date.embargoed.fl_str_mv | 2023-09-19T15:25:15Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10400.5/28649 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | platelets, double-spin, growth factors, orthobiologics, orthopedics, regenerative medicine, platelet-rich plasma Pain Management, Physical Medicine & Rehabilitation, Orthopedic |
| dc.title.fl_str_mv | A Simple Double-Spin Closed Method for Preparing Platelet-Rich Plasma |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Objective: To describe and analyze a new protocol for the extraction of platelet-rich plasma (PRP) for use in clinical practice and compare this technique with methods that have been previously described in the medical literature. Methods: Sixteen blood samples from healthy volunteers were collected. PRP was prepared using our new double-spin technique, consisting of successive centrifugation of blood samples with two different spins, without opening the container. Descriptive analysis of cell counts in baseline and PRP samples was undertaken. Comparison between cell and platelet count in baseline and PRP samples, as well as the statistical analysis, were done. Results: The mean platelet concentration ratio was 3.47 (SD: 0.85; 95% CI: 3.01-3.92; range: 2.48-5.71). The baseline whole blood platelet count correlated positively to the PRP platelet count (rP = 0.56; 95% CI: 0.09- 0.88; P = 0.023). The PRP was enriched for lymphocytes and monocytes but presented significantly lower counts of neutrophils and eosinophils in comparison to baseline. Conclusion: Results show a safe and easily reproducible method to obtain PRP for use in clinical daily practice. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorio.ulisboa.pt/bitstreams/c9a4d594-e8a4-4e7b-9d02-a8f5140861f0/download |
| id | ul_ba8aed51aedd003c500f3203b87bf784 |
| identifier.url.fl_str_mv | http://hdl.handle.net/10400.5/28649 |
| instacron_str | ul |
| institution | Universidade de Lisboa |
| instname_str | Universidade de Lisboa |
| language | eng |
| network_acronym_str | ul |
| network_name_str | Repositório da Universidade de Lisboa |
| oai_identifier_str | oai:repositorio.ulisboa.pt:10400.5/28649 |
| organization_str_mv | urn:organizationAcronym:ul |
| person_str_mv | Machado, Edilson S Soares, Fabiano P Yamaguchi, Roberta S Felipone, William K Meves, Robert Souza, Tais Amara C Topolniak, Roberto Caldas, José P Abreu, Ernani V Rabelo Neto, Luiz S Pinchemel, Pedro Vinicius S Bredemeier, Markus |
| publishDate | 2022 |
| reponame_str | Repositório da Universidade de Lisboa |
| repository_id_str | urn:repositoryAcronym:ul |
| service_str_mv | urn:repositoryAcronym:ul |
| spelling | engpt_PTObjective: To describe and analyze a new protocol for the extraction of platelet-rich plasma (PRP) for use in clinical practice and compare this technique with methods that have been previously described in the medical literature. Methods: Sixteen blood samples from healthy volunteers were collected. PRP was prepared using our new double-spin technique, consisting of successive centrifugation of blood samples with two different spins, without opening the container. Descriptive analysis of cell counts in baseline and PRP samples was undertaken. Comparison between cell and platelet count in baseline and PRP samples, as well as the statistical analysis, were done. Results: The mean platelet concentration ratio was 3.47 (SD: 0.85; 95% CI: 3.01-3.92; range: 2.48-5.71). The baseline whole blood platelet count correlated positively to the PRP platelet count (rP = 0.56; 95% CI: 0.09- 0.88; P = 0.023). The PRP was enriched for lymphocytes and monocytes but presented significantly lower counts of neutrophils and eosinophils in comparison to baseline. Conclusion: Results show a safe and easily reproducible method to obtain PRP for use in clinical daily practice.application/pdfpt_PTA Simple Double-Spin Closed Method for Preparing Platelet-Rich PlasmaMachado, Edilson SSoares, Fabiano PYamaguchi, Roberta SFelipone, William KMeves, RobertSouza, Tais Amara CTopolniak, RobertoCaldas, José PAbreu, Ernani VRabelo Neto, Luiz SPinchemel, Pedro Vinicius SBredemeier, MarkusHostingInstitutionOrganizationalRepositório Científico de Acesso Aberto da ULisboae-mailmailto:repositorio@reitoria.ulisboa.ptrepositorio@reitoria.ulisboa.ptDOIIsPartOf10.7759/cureus.208992023-09-19T15:25:15Z2022-01-032022-01-03T00:00:00ZHandlehttp://hdl.handle.net/10400.5/28649http://purl.org/coar/access_right/c_abf2open accessplatelets, double-spin, growth factors, orthobiologics, orthopedics, regenerative medicine, platelet-rich plasmaPain Management, Physical Medicine & Rehabilitation, Orthopedic191526 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal article2022-01-03http://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorio.ulisboa.pt/bitstreams/c9a4d594-e8a4-4e7b-9d02-a8f5140861f0/downloadCureus141 |
| spellingShingle | A Simple Double-Spin Closed Method for Preparing Platelet-Rich Plasma Machado, Edilson S platelets, double-spin, growth factors, orthobiologics, orthopedics, regenerative medicine, platelet-rich plasma Pain Management, Physical Medicine & Rehabilitation, Orthopedic |
| status | SINGLETON |
| subject.fl_str_mv | platelets, double-spin, growth factors, orthobiologics, orthopedics, regenerative medicine, platelet-rich plasma Pain Management, Physical Medicine & Rehabilitation, Orthopedic |
| title | A Simple Double-Spin Closed Method for Preparing Platelet-Rich Plasma |
| title_full | A Simple Double-Spin Closed Method for Preparing Platelet-Rich Plasma |
| title_fullStr | A Simple Double-Spin Closed Method for Preparing Platelet-Rich Plasma |
| title_full_unstemmed | A Simple Double-Spin Closed Method for Preparing Platelet-Rich Plasma |
| title_short | A Simple Double-Spin Closed Method for Preparing Platelet-Rich Plasma |
| title_sort | A Simple Double-Spin Closed Method for Preparing Platelet-Rich Plasma |
| topic | platelets, double-spin, growth factors, orthobiologics, orthopedics, regenerative medicine, platelet-rich plasma Pain Management, Physical Medicine & Rehabilitation, Orthopedic |
| topic_facet | platelets, double-spin, growth factors, orthobiologics, orthopedics, regenerative medicine, platelet-rich plasma Pain Management, Physical Medicine & Rehabilitation, Orthopedic |
| url | http://hdl.handle.net/10400.5/28649 |
| visible | 1 |