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The effects of rockrose extract feed inclusion on the wool proteome of portuguese White Merinos

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Resumo:This study was carried out with the objective of evaluating the effect of feeding extract of rockrose (Cistus ladanifer L.), a perennial shrub commonly found in the marginal regions of Portugal and rich in condensed tannins (CT), and its effect on the wool proteome of Portuguese White Merinos. Twenty-four Portuguese White Merinos lambs were fed a diet restricted in protein and composed of grass hay (15%) and concentrate (85%), where they were distributed in three experimental groups: control group, composed of 16% crude protein (CP); diet restricted to 12% crude protein (RP) and diet restricted to 12% crude protein and treated with 1.5% rockrose extract (RPCT). After the 5-week experimental period, samples of wool present in an area of approximately 100cm² in the anterior region of the head were collected from each animal and used for subsequent analysis of proteomics based on liquid chromatography - mass spectrometry (LC-MS). The proteomic method identified 110 proteins in the wool fibre. Of this total, 4 showed significance (P<0.05) and could be classified as structural proteins, with three Type I keratins (Keratin 36, Keratin Type I and Keratin 10) and one Keratin Associated Protein (KAP9.2). The differential accumulation results showed that the RPCT group decreased the expression of these proteins in relation to the Control and RP groups. Except for the K36 protein, which had a 19% higher expression for the RPCT group compared to the other two groups. We conclude that the rockrose extract has a minor effect on the wool proteome that affect specifically the parts of the wool fibre where these proteins are located with putative changes in fibre structure and properties. Finally, further microscopy and wool quality traits studies are still needed to confirm and explain these changes
Autores principais:Pizol, José Vitor
Assunto:rockrose condensed tannins wool fibre wool proteomics White Merino
Ano:2020
País:Portugal
Tipo de documento:dissertação de mestrado
Tipo de acesso:acesso aberto
Instituição associada:Universidade de Lisboa
Idioma:inglês
Origem:Repositório da Universidade de Lisboa

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