Publicação
Design and functionalization of a µpad for the enzymatic determination of nitrate in urine
| Resumo: | In this work, the design of a microfluidic paper-based analytical device (µPAD) for the quantification of nitrate in urine samples was described. Nitrate monitoring is highly relevant due to its association to some diseases and health conditions. The nitrate determination was achieved by combining the selectivity of the nitrate reductase enzymatic reaction with the colorimetric detection of nitrite by the well-known Griess reagent. For the optimization of the nitrate determination µPAD, several variables associated with the design and construction of the device were studied. Furthermore, the interference of the urine matrix was evaluated, and stability studies were performed, under different conditions. The developed µPAD enabled us to obtain a limit of detection of 0.04 mM, a limit of quantification of 0.14 mM and a dynamic concentration range of 0.14–1.0 mM. The designed µPAD proved to be stable for 24 h when stored at room temperature in air or vacuum atmosphere, and 60 days when stored in vacuum at −20◦C. The accuracy of the nitrate µPAD measurements was confirmed by analyzing four certified samples (prepared in synthetic urine) and performing recovery studies using urine samples. |
|---|---|
| Autores principais: | Ferreira, Francisca T. S. M. |
| Outros Autores: | Mesquita, Raquel B. R.; Rangel, António O. S. S. |
| Assunto: | Griess reaction Hydrophilic membrane Microfluidic paper-based device Nitrate reductase Urine samples |
| Ano: | 2021 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade Católica Portuguesa |
| Idioma: | inglês |
| Origem: | Veritati - Repositório Institucional da Universidade Católica Portuguesa |
| _version_ | 1866895369761193984 |
|---|---|
| author | Ferreira, Francisca T. S. M. |
| author2 | Mesquita, Raquel B. R. Rangel, António O. S. S. |
| author2_role | author author |
| author_facet | Ferreira, Francisca T. S. M. Mesquita, Raquel B. R. Rangel, António O. S. S. |
| author_role | author |
| contributor_name_str_mv | Veritati |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Ferreira, Francisca T. S. M.\",\"Person.identifier.orcid\":\"0000-0002-3921-3973\"},{\"Person.name\":\"Mesquita, Raquel B. R.\",\"Person.identifier.orcid\":\"0000-0001-7745-1860\"},{\"Person.name\":\"Rangel, António O. S. S.\",\"Person.identifier.orcid\":\"0000-0002-6486-8947\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Veritati |
| datacite.creators.creator.creatorName.fl_str_mv | Ferreira, Francisca T. S. M. Mesquita, Raquel B. R. Rangel, António O. S. S. |
| datacite.date.Accepted.fl_str_mv | 2021-11-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2021-11-04T16:20:22Z |
| datacite.date.embargoed.fl_str_mv | 2021-11-04T16:20:22Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Griess reaction Hydrophilic membrane Microfluidic paper-based device Nitrate reductase Urine samples |
| datacite.titles.title.fl_str_mv | Design and functionalization of a µpad for the enzymatic determination of nitrate in urine |
| dc.contributor.none.fl_str_mv | Veritati |
| dc.creator.none.fl_str_mv | Ferreira, Francisca T. S. M. Mesquita, Raquel B. R. Rangel, António O. S. S. |
| dc.date.Accepted.fl_str_mv | 2021-11-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2021-11-04T16:20:22Z |
| dc.date.embargoed.fl_str_mv | 2021-11-04T16:20:22Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10400.14/35815 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Griess reaction Hydrophilic membrane Microfluidic paper-based device Nitrate reductase Urine samples |
| dc.title.fl_str_mv | Design and functionalization of a µpad for the enzymatic determination of nitrate in urine |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | In this work, the design of a microfluidic paper-based analytical device (µPAD) for the quantification of nitrate in urine samples was described. Nitrate monitoring is highly relevant due to its association to some diseases and health conditions. The nitrate determination was achieved by combining the selectivity of the nitrate reductase enzymatic reaction with the colorimetric detection of nitrite by the well-known Griess reagent. For the optimization of the nitrate determination µPAD, several variables associated with the design and construction of the device were studied. Furthermore, the interference of the urine matrix was evaluated, and stability studies were performed, under different conditions. The developed µPAD enabled us to obtain a limit of detection of 0.04 mM, a limit of quantification of 0.14 mM and a dynamic concentration range of 0.14–1.0 mM. The designed µPAD proved to be stable for 24 h when stored at room temperature in air or vacuum atmosphere, and 60 days when stored in vacuum at −20◦C. The accuracy of the nitrate µPAD measurements was confirmed by analyzing four certified samples (prepared in synthetic urine) and performing recovery studies using urine samples. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorio.ucp.pt/bitstreams/74f82602-bda4-476f-93e9-0b805617de3c/download |
| id | veritati_b3e0c1937628e5b1ad4e84afaf23cb6e |
| identifier.url.fl_str_mv | http://hdl.handle.net/10400.14/35815 |
| instacron_str | ucp |
| institution | Universidade Católica Portuguesa |
| instname_str | Universidade Católica Portuguesa |
| language | eng |
| network_acronym_str | veritati |
| network_name_str | Veritati - Repositório Institucional da Universidade Católica Portuguesa |
| oai_identifier_str | oai:repositorio.ucp.pt:10400.14/35815 |
| organization_str_mv | urn:organizationAcronym:ucp |
| person_str_mv | Ferreira, Francisca T. S. M. Ferreira, Francisca T. S. M. https://www.ciencia-id.pt/7F1F-5904-A950 7F1F-5904-A950 http://orcid.org/0000-0002-3921-3973 0000-0002-3921-3973 Mesquita, Raquel B. R. Mesquita, Raquel B. R. https://www.ciencia-id.pt/EB1D-DEE3-0B60 EB1D-DEE3-0B60 http://orcid.org/0000-0001-7745-1860 0000-0001-7745-1860 Rangel, António O. S. S. Rangel, António O. S. S. https://www.ciencia-id.pt/D61D-7CFB-178A D61D-7CFB-178A http://orcid.org/0000-0002-6486-8947 0000-0002-6486-8947 |
| publishDate | 2021 |
| reponame_str | Veritati - Repositório Institucional da Universidade Católica Portuguesa |
| repository_id_str | urn:repositoryAcronym:veritati |
| service_str_mv | urn:repositoryAcronym:veritati |
| spelling | engpt_PTIn this work, the design of a microfluidic paper-based analytical device (µPAD) for the quantification of nitrate in urine samples was described. Nitrate monitoring is highly relevant due to its association to some diseases and health conditions. The nitrate determination was achieved by combining the selectivity of the nitrate reductase enzymatic reaction with the colorimetric detection of nitrite by the well-known Griess reagent. For the optimization of the nitrate determination µPAD, several variables associated with the design and construction of the device were studied. Furthermore, the interference of the urine matrix was evaluated, and stability studies were performed, under different conditions. The developed µPAD enabled us to obtain a limit of detection of 0.04 mM, a limit of quantification of 0.14 mM and a dynamic concentration range of 0.14–1.0 mM. The designed µPAD proved to be stable for 24 h when stored at room temperature in air or vacuum atmosphere, and 60 days when stored in vacuum at −20◦C. The accuracy of the nitrate µPAD measurements was confirmed by analyzing four certified samples (prepared in synthetic urine) and performing recovery studies using urine samples.application/pdfpt_PTDesign and functionalization of a µpad for the enzymatic determination of nitrate in urinePersonalFerreira, Francisca T. S. M.DSpacehttp://dspace.org/items/dbd4a93d-f402-4d17-ba5e-661210af54b8DSpacehttp://dspace.org/items/dbd4a93d-f402-4d17-ba5e-661210af54b8FerreiraFranciscaCiência IDhttps://www.ciencia-id.pt7F1F-5904-A950ORCIDhttp://orcid.org0000-0002-3921-3973PersonalMesquita, Raquel B. R.DSpacehttp://dspace.org/items/f89887f1-4d1b-438b-8f03-27d9bda1de7bDSpacehttp://dspace.org/items/f89887f1-4d1b-438b-8f03-27d9bda1de7bMesquitaRaquelCiência IDhttps://www.ciencia-id.ptEB1D-DEE3-0B60ORCIDhttp://orcid.org0000-0001-7745-1860Researcher IDhttps://www.researcherid.comJ-4630-2013Scopus Author IDhttps://www.scopus.com7005556626Scopus Author IDhttps://www.scopus.com7005556626PersonalRangel, António O. S. S.DSpacehttp://dspace.org/items/467be022-fd9c-4549-aa48-48ebcc1098ebDSpacehttp://dspace.org/items/467be022-fd9c-4549-aa48-48ebcc1098ebSantos Silva RangelAntónio OsmaroCiência IDhttps://www.ciencia-id.ptD61D-7CFB-178AORCIDhttp://orcid.org0000-0002-6486-8947Scopus Author IDhttps://www.scopus.com7004827387HostingInstitutionOrganizationalVeritatie-mailmailto:repositorio@ucp.ptrepositorio@ucp.ptISSNIsPartOf1420-3049DOIIsPartOf10.3390/molecules262163552021-11-04T16:20:22Z2021-11-012021-11-01T00:00:00ZHandlehttp://hdl.handle.net/10400.14/35815http://purl.org/coar/access_right/c_abf2open accessGriess reactionHydrophilic membraneMicrofluidic paper-based deviceNitrate reductaseUrine samples1162593 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal article2021-11-01http://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorio.ucp.pt/bitstreams/74f82602-bda4-476f-93e9-0b805617de3c/downloadMolecules2621 |
| spellingShingle | Design and functionalization of a µpad for the enzymatic determination of nitrate in urine Ferreira, Francisca T. S. M. Griess reaction Hydrophilic membrane Microfluidic paper-based device Nitrate reductase Urine samples |
| status | SINGLETON |
| subject.fl_str_mv | Griess reaction Hydrophilic membrane Microfluidic paper-based device Nitrate reductase Urine samples |
| title | Design and functionalization of a µpad for the enzymatic determination of nitrate in urine |
| title_full | Design and functionalization of a µpad for the enzymatic determination of nitrate in urine |
| title_fullStr | Design and functionalization of a µpad for the enzymatic determination of nitrate in urine |
| title_full_unstemmed | Design and functionalization of a µpad for the enzymatic determination of nitrate in urine |
| title_short | Design and functionalization of a µpad for the enzymatic determination of nitrate in urine |
| title_sort | Design and functionalization of a µpad for the enzymatic determination of nitrate in urine |
| topic | Griess reaction Hydrophilic membrane Microfluidic paper-based device Nitrate reductase Urine samples |
| topic_facet | Griess reaction Hydrophilic membrane Microfluidic paper-based device Nitrate reductase Urine samples |
| url | http://hdl.handle.net/10400.14/35815 |
| visible | 1 |