Publicação
Sphingobium vermicomposti sp. nov., isolated from vermicompost
| Resumo: | Strain VC-230T was isolated from homemade vermicompost produced from kitchen waste. The isolate was a Gram-negative-staining, catalase- and oxidase-positive, motile rod-shaped bacterium able to grow at 15–37 6C and pH 6–8. On the basis of 16S rRNA gene sequence analysis, strain VC-230T was determined to belong to the family Sphingomonadaceae by its clustering with type strains of the genus Sphingobium, with Sphingobium chlorophenolicum ATCC 33790T (97.7 %) and Sphingobium herbicidovorans DSM 11019T (97.4 %) as its closest neighbours. The polar lipid pattern, the presence of spermidine and ubiquinone 10, the predominance of the cellular fatty acids C18 : 1v7c/9t/12t, C16 : 1v7c and C16 : 0 and the G+C content of the genomic DNA supported the affiliation of this organism to the genus Sphingobium. The phylogenetic, chemotaxonomic, phenotypic and DNA–DNA hybridization analyses verify that strain VC-230T represents a novel species, for which the name Sphingobium vermicomposti sp. nov. is proposed. The type strain is VC-230T (5CCUG 55809T 5DSM 21299T). |
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| Autores principais: | Vaz-Moreira, Ivone |
| Outros Autores: | Faria, Cátia; Lopes, Ana R.; Svensson, Liselott; Falsen, Enevold; Moore, Edward R. B.; Ferreira, António C. Silva; Nunes, Olga C.; Manaia, Célia M. |
| Ano: | 2009 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso restrito |
| Instituição associada: | Universidade Católica Portuguesa |
| Idioma: | inglês |
| Origem: | Veritati - Repositório Institucional da Universidade Católica Portuguesa |
| Resumo: | Strain VC-230T was isolated from homemade vermicompost produced from kitchen waste. The isolate was a Gram-negative-staining, catalase- and oxidase-positive, motile rod-shaped bacterium able to grow at 15–37 6C and pH 6–8. On the basis of 16S rRNA gene sequence analysis, strain VC-230T was determined to belong to the family Sphingomonadaceae by its clustering with type strains of the genus Sphingobium, with Sphingobium chlorophenolicum ATCC 33790T (97.7 %) and Sphingobium herbicidovorans DSM 11019T (97.4 %) as its closest neighbours. The polar lipid pattern, the presence of spermidine and ubiquinone 10, the predominance of the cellular fatty acids C18 : 1v7c/9t/12t, C16 : 1v7c and C16 : 0 and the G+C content of the genomic DNA supported the affiliation of this organism to the genus Sphingobium. The phylogenetic, chemotaxonomic, phenotypic and DNA–DNA hybridization analyses verify that strain VC-230T represents a novel species, for which the name Sphingobium vermicomposti sp. nov. is proposed. The type strain is VC-230T (5CCUG 55809T 5DSM 21299T). |
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