Author(s): Fraqueza, M.J. ; Rocha, J.M. ; Laranjo, M. ; Potes, M.E. ; Fialho, A.R. ; Fernandes, M.J. ; Fernandes, M.H. ; Barreto, A. ; Semedo-Lemsaddek, T. ; Elias, M.
Date: 2019
Persistent ID: http://hdl.handle.net/10174/26179
Origin: Repositório Científico da Universidade de Évora
Subject(s): antibiotic resistance profile; food safety; rep-PCR fingerprinting; Staphylococcus
The microbiota of traditional dry-cured sausages and industrial environment was assessed to characterize the diversity of coagulase-negative staphylococci (CNS), and establish potential relationships with hygiene level or technological characteristics. Eight processing units from South Portugal were audited according to a checklist of requirements. Environmental and products’ samples at different production stages were evaluated regarding hygiene and safety criteria. CNS were recovered, characterized, and their potential use as starters evaluated. Low genetic diversity was observed for Staphylococcus xylosus, whereas Staphylococcus equorum showed diverse genetic profiles. Staphylococcus xylosus predominated in products with a long period of cold smoking, Staphylococcus saprophyticus in products with a long period of hot smoking, Staphylococcus epidermidis in products with a short period of cold smoking, and S. equorum in nonsmoked products. Most S. xylosus were resistant to tetracycline, whereas S. equorum were susceptible. Antibioresistance restricted the selection of starters due to safety recommendations
