Author(s): Panserat, Stephane ; Plagnes-Juan, Elisabeth ; Gazzola, Elsa ; Palma, Mariana ; Magnoni, Leonardo J. ; Marandel, Lucie ; Viegas, Ivan
Date: 2020
Persistent ID: https://hdl.handle.net/10316/106124
Origin: Estudo Geral - Universidade de Coimbra
Subject(s): glycerol metabolism; fish nutrition; genomics; gene expression; rainbow trout; aquaculture
Glycerol metabolism in rainbow trout is poorly studied even though it is at the interface between lipid and glucose metabolism. Moreover, glycerol can be an important ingredient in new aquafeed formulation to decrease the catabolism of dietary amino acids. Thus, the present study aimed to characterize for the first time the different genes coding for key enzymes and proteins involved in hepatic glycerol metabolism. From the trout genomes, all the paralogous genes coding for glycerol transport (aqp9b), glycerol kinase (gk2a and gk5), glycerol-3-phosphate phosphatase (pgp), and glycerol-3-phosphate dehydrogenase (gpd1a, gpd1b, and gpd1c) were identified. The ontogenesis determined that the capacity to metabolize glycerol begins with the apparition of the liver during the development (stage 22) and are more expressed at the endogenous-exogenous feeding period (stage 35). The postprandial regulation of the expression of these genes in juvenile trout showed that the postprandial peak of expression is between 4 and 24 h after the last meal for many of the genes, demonstrating that glycerol metabolism could be nutritionally regulated at a molecular level. However, surprisingly, no regulation of the mRNA abundance for the glycerol metabolism-related genes by different levels of dietary glycerol (0, 2.5, and 5%) have been detected, showing that hepatic glycerol metabolism is poorly regulated at a molecular level by dietary glycerol in rainbow trout juveniles.
This work was supported by Fundação para a Ciência e Tecnologia (FCT; Portugal) through national funds with cofunding from ERDF/FEDER, within the PT2020 Partnership Agreement, and COMPETE 2020: research grant to IV (POCI-01-0145-FEDER-016828 - PTDC/CVT-NUT/2851/2014); individual grant to MP through Centro2020 (ReNATURE; Centro-01-0145-FEDER-000007); and structural funds to Center for Neuroscience and Cell Biology (UID/NEU/04539/2013) and Centre for Functional Ecology (UID/BIA/04004/2019) and Interdisciplinary Centre of Marine and Environmental Research (UID/Multi/04423/2019). The research about gene ontogenesis was internally funded by the European Commission (European project FP7-KBBE-2011-5, ARRAINA project no. 288925, Advanced Research Initiatives for Nutrition 1241 and Aquaculture)
