Autor(es):
Cotas, João ; Marques, Vanda ; Afonso, Marta B. ; Rodrigues, Cecília M. P. ; Pereira, Leonel
Data: 2020
Identificador Persistente: https://hdl.handle.net/10316/106352
Origem: Estudo Geral - Universidade de Coimbra
Assunto(s): antitumour activity; carrageenan; colorectal cancer; cancer stem cells; Antineoplastic Agents; Biological Products; Carrageenan; Cell Line, Tumor; Cell Survival; Colorectal Neoplasms; Humans; Inhibitory Concentration 50; Neoplastic Stem Cells; Rhodophyta; Seaweed
Descrição
Gigartina pistillata is a red seaweed common in Figueira da Foz, Portugal. Here, the antitumour potential of G. pistillata carrageenan, with a known variable of the life cycle, the female gametophyte (FG) and tetrasporophyte (T) was evaluated against colorectal cancer stem cell (CSC) -enriched tumourspheres. FTIR-ATR analysis of G. pistillata carrageenan extracts indicated differences between life cycle phases, being FG a κ/ι hybrid carrageenan and T a ʎ/ξ hybrid. Both carrageenan extracts presented IC50 values inferior to 1 μg/mL in HT29-derived CSC-enriched tumourspheres, as well as reduced tumoursphere area. The two extracts were also effective at reducing cellular viability in SW620- and SW480-derived tumourspheres. These results indicate that carrageenans extracted from two G. pistillata life cycle phases have antitumour potential against colorectal cancer stem-like cells, specially the T carrageenan.
