Autor(es):
Floriddia, Elisa M. ; Lourenço, Tânia ; Zhang, Shupei ; van Bruggen, David ; Hilscher, Markus M. ; Kukanja, Petra ; Gonçalves Dos Santos, João P. ; Altınkök, Müge ; Yokota, Chika ; Llorens-Bobadilla, Enric ; Mulinyawe, Sara B ; Grãos, Mário ; Sun, Lu O. ; Frisén, Jonas ; Nilsson, Mats ; Castelo-Branco, Gonçalo
Data: 2020
Identificador Persistente: https://hdl.handle.net/10316/106507
Origem: Estudo Geral - Universidade de Coimbra
Assunto(s): Animals; Axons; Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; Biomarkers; Cell Lineage; Corpus Callosum; Encephalomyelitis, Autoimmune, Experimental; Female; Gene Expression Profiling; Mice, Inbred C57BL; Mice, Inbred CBA; Mice, Knockout; Mice, Transgenic; Oligodendroglia; Single-Cell Analysis; Spinal Cord; Spinal Cord Injuries
Descrição
Mature oligodendrocytes (MOLs) show transcriptional heterogeneity, the functional consequences of which are unclear. MOL heterogeneity might correlate with the local environment or their interactions with different neuron types. Here, we show that distinct MOL populations have spatial preference in the mammalian central nervous system (CNS). We found that MOL type 2 (MOL2) is enriched in the spinal cord when compared to the brain, while MOL types 5 and 6 (MOL5/6) increase their contribution to the OL lineage with age in all analyzed regions. MOL2 and MOL5/6 also have distinct spatial preference in the spinal cord regions where motor and sensory tracts run. OL progenitor cells (OPCs) are not specified into distinct MOL populations during development, excluding a major contribution of OPC intrinsic mechanisms determining MOL heterogeneity. In disease, MOL2 and MOL5/6 present different susceptibility during the chronic phase following traumatic spinal cord injury. Our results demonstrate that the distinct MOL populations have different spatial preference and different responses to disease.
