Author(s): Pasquel Reátegui, José Luis, 1985- ; Machado, Ana Paula da Fonseca, 1988- ; Rezende, Camila Alves de, 1980- ; Martínez, Julian, 1976-
Date: 2014
Persistent ID: https://hdl.handle.net/20.500.12733/225
Origin: Oasisbr
Subject(s): Amora; Microscopia eletrônica de varredura; Ultrassom; Mulberry; Scanning electron microscopy; Ultrasonics; Food wastes; Supercritical fluid extraction; Artigo original
Agradecimentos: The authors wish to thank CAPES (Grant no. 2952/2011), by thegranting of the scholarship and funding this research project andfinancial aid, FAPESP (2012/22119-7 and 2013/02203-6) and CNPq (473342/2011-1) for financing research projects, and the BrazilianNational Nanotechnology Laboratory (LNNano) for the experimen-tal support
Abstract: Supercritical carbon dioxide extraction (SFE) was performed to recover bioactive components from blackberry (Rubus sp.) industrial residues. Ultrasound was applied during the extractions in order to enhance rate and yield. Moreover, water and ethanol at different proportions were used as cosolvents to improve the extraction of polar compounds from the residues. The extraction global yields were measured at all performed conditions (pressure, temperature, ultrasound power and cosolvent). The extracts were evaluated in terms of their antioxidant activity, measured using two methods, phenolic content, monomeric anthocyanins, and anthocyanin profile. The application of ultrasound in SFE helped increasing the extraction rate at the beginning of the process, which could be observed on the extraction curves at 15 MPa, the lowest pressure applied. Scanning electron microscopy (FESEM) was used to analyze the blackberry bagasse undergoing SFE with and without ultrasounds and showed that ultrasound disturbs the cell walls, enhancing the release of the extractable compounds. The extracts have shown high antioxidant activity and phenolic contents when obtained at higher temperatures. Regarding anthocyanins, the use of water as cosolvent resulted in a significant increase. Four major anthocyanins were identified and quantified by ultra performance liquid chromatography (UPLC)
CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ
COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPES
FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP
Fechado
