Author(s): Oldoni, Tatiane Luiza Cadorin ; Cabral, Ingridy Simone Ribeiro ; d’Arce, Marisa Aparecida Bismara Regitano ; Rosalen, Pedro Luiz ; Ikegaki, Masaharu ; Nascimento, Andréa Mendes do ; Alencar, Severino Matias de
Date: 2012
Origin: Oasisbr
Subject(s): Antioxidant activity; Brazilian propolis; Antimicrobial activity; Isoflavonoids; Chalcone
Activity-directed fractionation and purification processes were employed to identify isoflavonoids with antioxidant and antimicrobial activities from Brazilian red propolis. Crude propolis was extracted with ethanol (80%, v/v) and fractioned by liquid–liquid extraction technique using hexane and chloroform. Since chloroform fraction showed strong antioxidant and antimicrobial activities it was purified and isolated using various chromatographic techniques. Comparing our spectral data (UV, NMR, and mass spectrometry) with values found in the literature, we identified two bioactiveisoflavonoids (vestitol and neovestitol), together with one chalcone (isoliquiritigenin). Vestitol presented higher antioxidant activity against β-carotene consumption than neovestitol. The antimicrobial activity of these three compounds against Staphylococcus aureus, Streptococcus mutans, and Actinomyces naeslundii was evaluated and we concluded that isoliquiritigenin was the most active one with lower MIC, ranging from 15.6 to 62.5 μg/mL. Our results showed that Brazilian red propolis has biologically active isoflavonoids that may be used as a mild antioxidant and antimicrobial for food preservation.
