Autor(es): Kalapothakis, Evanguedes ; Jardim, S. ; Magalhães, Ana Cristina Ribeiro ; Mendes, Thais Melo ; Marco, Luiz de ; Afonso, Luís Carlos Crocco ; Olórtegui, Carlos Delfin Chávez
Data: 2015
Origem: Oasisbr
Assunto(s): Screening; Expression library; Genome; Proteome
The present report describes the use of ELISA with cDNA expression libraries in the identification of immunogenic proteins. The methodology described was applied using libraries constructed with mRNA isolated from Tityus serrulatus and Tityus bahiensis venom glands. In addition we describe for the first time the sequence of a neurotoxin from Tityus bahiensis venom gland named TbTx5 whose amino acid sequencing showed 93% similarity with the Tityus bahiensis TbTx IV-5 neurotoxin. The methodology described can be used for the generation of an immunogenic bank in order to contribute to genome and proteome projects.
