Coxsackievirus B5 induced apoptosis of HeLa cells : effects on p53 and SUMO.

• Autor(es): Gomes, Rogério ; Cota, Renata Guerra de Sá ; Arruda, Eurico
• Data: 2015
• Origem: Oasisbr
• Assunto(s): Coxsackievirus B5; Apoptosis
• Tipo de Documento: Artigo científico
• Idioma: Inglês

Descrição

Coxsackievirus B5 (CVB5), a human enterovirus of the family Picornaviridae, is a frequent cause of acute and chronic human diseases. The pathogenesis of enteroviral infections is not completely understood, and the fate of the CVB5-infected cell has a pivotal role in this process. We have investigated the CVB5-induced apoptosis of HeLa cells and found that it happens by the intrinsic pathway by a mechanism dependent on the ubiquitin–proteasome system, associated with nuclear aggregation of p53. Striking redistribution of both SUMO and UBC9 was noted at 4 h post-infection, simultaneously with a reduction in the levels of the ubiquitin-ligase HDM2. Taken together, these results suggest that CVB5 infection of HeLa cells elicit the intrinsic pathway of apoptosis by MDM2 degradation and p53 activation, destabilizing protein sumoylation, by a mechanism that is dependent on a functional ubiquitin–proteasome system.