Autor(es): Silva-Fernandes, Talita ; Duarte, Luís C. ; Carvalheiro, Florbela ; Marques, Susana ; Loureiro-Dias, M. Conceição ; Fonseca, César ; Gírio, Francisco
Data: 2015
Identificador Persistente: http://hdl.handle.net/10400.9/2905
Origem: Repositório do LNEG
Assunto(s): Lignocellulosic biomass; Autohydrolysis; Enzymatic hydrolysis; Biorefinery; Eucalyptus residues; Wheat Straw; Olive residues
This work proposes the biorefining of eucalyptus residues (ER), wheat straw (WS) and olive tree pruning (OP) combining hydrothermal pretreatment (autohydrolysis) with acid post-hydrolysis of the liquid fraction and enzymatic hydrolysis of the solid fraction towards maximal recovery of monosaccharides from those lignocellulose materials. Autohydrolysis of ER, WS and OP was performed under non-isothermal conditions (195–230 C) and the non-cellulosic saccharides were recovered in the liquid fraction while cellulose and lignin remained in the solid fraction. The acid post-hydrolysis of the soluble oligosaccharides was studied by optimizing sulfuric acid concentration (1–4% w/w) and reaction time (10–60 min), employing a factorial (22) experimental design. The solids resulting from pretreatment were submitted to enzymatic hydrolysis by applying commercial cellulolytic enzymes Celluclast1.5 L and Novozyme 188 (0.225 and 0.025 g/g solid, respectively). This strategy provides high total monosaccharide recovery or high glucose recovery from lignocellulosic materials, depending on the autohydrolysis conditions applied.
