Author(s):
Silva, Daniela Caroline Barbosa da ; Lima, Helena Maria Reinaldo ; Silva, Francisco Laurindo da ; Sousa, Francisco das Chagas Araújo ; Silva, Wenderson Costa da ; Reis, Liana Cynthia de Macedo ; Sousa, Marcos André Arrais de ; Pimentel, Alice de Castro Cruz ; Furtado, Daniel Rodrigues ; Rodrigues, Augusto Cesar Evelin ; Alves, Adrielle Martins Monteiro ; Rodrigues, Renan Paraguassu de Sá ; Monteiro, Amanda Laurindo
Date: 2021
Origin: Oasisbr
Subject(s): Propóleos; Actividad antibacterial; Fitoterápico.; Actividad antibacterial; Fitoterápico; Propóleos.; Antibacterials; Propolis; Antibacterial activity; Phytotherap.; Herbal Medicines; Propolis.; Própolis; Antibacterianos; Fitoterápicos.; Antibacterianos; Fitoterápicos; Própolis.
Description
Natural-source products are being studied to preserve human health, including to prevent oral diseases, because industrialized drugs can cause bacterial resistance and adverse effects. Red propolis, a resinous and complex substance, of natural origin produced by bees, has therapeutic properties such as antimicrobial and anti-inflammatory activity. The objective of this work was to verify the antibacterial effect of red propolis in the control of bacteria in the oral cavity and the specific objectives were to compare the effect of propolis with the effect of chlorhexidine and determine in which bacterial strains propolis was more effective. Strains of Escherichia coli (ATCC-25922), Enterococcus faecalis (ATCC-29212) and Staphylococcus aureus (ATCC-25923) were used. The antimicrobial activity of propolis was determined by the agar diffusion method with the well technique. The wells were filled with 40 µL of 0.12% chlorhexidine solutions (positive control), red propolis in concentrations of 12%, 6%, 3%, 1.2%, 0.12%, 0.012% and distilled water (negative control). Tests were performed in triplicate and statistical analysis was performed using Student's t test. Data were organized into tables and graphs to demonstrate the results obtained. Values of p <0.05 were considered significant. It was observed that propolis in general showed similar effectiveness to chlorhexidine in Gram-positive bacteria. It can be concluded that red propolis in its varied concentrations had antimicrobial effects. Its effectiveness was as good as that of chlorhexidine in Gram-positive bacteria and in Gram-negative bacteria there was no inhibitory effect.
Natural-source products are being studied to preserve human health, including to prevent oral diseases, because industrialized drugs can cause bacterial resistance and adverse effects. Red propolis, a resinous and complex substance, of natural origin produced by bees, has therapeutic properties such as antimicrobial and anti-inflammatory activity. The objective of this work was to verify the antibacterial effect of red propolis in the control of bacteria in the oral cavity and the specific objectives were to compare the effect of propolis with the effect of chlorhexidine and determine in which bacterial strains propolis was more effective. Strains of Escherichia coli (ATCC-25922), Enterococcus faecalis (ATCC-29212) and Staphylococcus aureus (ATCC-25923) were used. The antimicrobial activity of propolis was determined by the agar diffusion method with the well technique. The wells were filled with 40 µL of 0.12% chlorhexidine solutions (positive control), red propolis in concentrations of 12%, 6%, 3%, 1.2%, 0.12%, 0.012% and distilled water (negative control ). Tests were performed in triplicate and statistical analysis was performed using Student's t test. Data were organized into tables and graphs to demonstrate the results obtained. Values of p <0.05 were considered significant. It was observed that propolis in general showed similar effectiveness to chlorhexidine in Gram-positive bacteria. It can be concluded that red propolis in its varied concentrations had antimicrobial effects. Its effectiveness was as good as that of chlorhexidine in Gram-positive bacteria and in Gram-negative bacteria there was no inhibitory effect.
Produtos de fonte de naturais estão sendo estudado para preservar a saúde humana, inclusive para prevenir doenças orais, pelo fato de medicamentos industrializados poderem acarretar resistência bacteriana e efeitos adversos. A própolis vermelha uma substância resinosa e complexa, de origem natural produzida pelas abelhas, têm propriedades terapêuticas, como atividade antimicrobiana e anti-inflamatória. O objetivo deste trabalho foi verificar o efeito antibacteriano da própolis vermelha no controle de bactérias da cavidade oral e os objetivos específicos foram comparar o efeito da própolis com efeito da clorexidina e determinar em quais cepas bacterianas a própolis apresentava maior eficácia. Utilizou-se cepas de Escherichia coli (ATCC-25922), Enterococcus faecalis (ATCC-29212) e Staphylococcus aureus (ATCC-25923). A atividade antimicrobiana da própolis foi determinada pelo método de difusão em ágar com a técnica de poços. Os poços foram preenchidos com 40 µL das soluções de clorexidina 0,12% (controle positivo), própolis vermelha em concentrações de 12%, 6%, 3%, 1.2%, 0,12%, 0,012% e água destilada (controle negativo). Os testes aconteceram em triplicata e a análise estatística foi realizada através do teste T de Student. Os dados foram organizados em tabelas e gráficos para a demonstração dos resultados obtidos. Considerou-se como significantes valores de p <0,05. Observou-se que a própolis de maneira geral apresentou efetividade semelhante ao da clorexidina em bactérias Gram-positivas. Pode-se concluir que a própolis vermelha em suas variadas concentrações, apresentaram efeitos antimicrobianos. Sua efetividade foi tão boa quanto o da clorexidina em bactérias Gram-positivas e em bactérias Gram-negativas não houve efeito inibitório.
