Author(s):
Furini, Adriana Ant?nia da Cruz ; Cassiano, Gustavo Capatti ; Capobianco, Marcela Petrolini ; Santos, Sidney Emanuel Batista dos ; Machado, Ricardo Luiz Dantas
Date: 2017
Origin: Oasisbr
Subject(s): Mal?ria Vivax / gen?tica; Polimorfismo Gen?tico / gen?tica; Frequ?ncia do Gene / gen?tica; Grupo com Ancestrais do Continente Africano; Grupo com Ancestrais do Continente Europeu; Grupo com Ancestrais Nativos do Continente Americano; Polimorfismo de Fragmento de Restri??o / gen?tica; Rea??o em Cadeia da Polimerase / m?todos; Ecossistema Amaz?nico; Brasil (BR)
Description
College of Medicine of S?o Jos? do Rio Preto. Department of Dermatologic, Infectious, and Parasitic Diseases. S?o Jos? do Rio Preto, SP, Brazil / University Center of Rio Preto. S?o Jos? do Rio Preto, SP, Brazil.
University of Campinas. Department of Genetics, Evolution and Bioagents. Laboratory of Tropical Diseases - Prof. Luiz Jacintho da Silva. Campinas, SP, Brazil.
S?o Paulo State University. Department of Biology. S?o Jos? do Rio Preto, SP, Brazil.
Federal University of Par?. Laboratory of Human and Medical Genetics. Bel?m, PA, Brazil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Laborat?rio de Pesquisa B?sica em Mal?ria. Ananindeua, PA, Brasil / S?o Paulo State University. Department of Biology. S?o Jos? do Rio Preto, SP, Brazil.
Polymorphisms in cytokine genes can alter the production of these proteins and consequently affect the immune response. The trihybrid heterogeneity of the Brazilian population is characterized as a condition for the use of ancestry informative markers. The objective of this study was to evaluate the frequency of -1031T>C, -308G>A and -238G>A TNFA, +874 A>T IFNG and -819C>T, and -592C>A IL10 gene polymorphisms and their association with malaria vivax and genomic ancestry. Samples from 90 vivax malaria-infected individuals and 51 noninfected individuals from northern Brazil were evaluated. Genotyping was carried out by using ASO-PCR or PCR/RFLP. The genomic ancestry of the individuals was classified using 48 insertion/deletion polymorphism biallelic markers. There were no differences in the proportions of African, European, and Native American ancestry between men and women. No significant association was observed for the allele and genotype frequencies of the 6 SNPs between malaria-infected and noninfected individuals. However, there was a trend toward decreasing the frequency of individuals carrying the TNF-308A allele with the increasing proportion of European ancestry. No ethnic-specific SNPs were identified, and there was no allelic or genotype association with susceptibility or resistance to vivax malaria. Understanding the genomic mechanisms by which ancestry influences this association is critical and requires further study.
