Autor(es):
Furini, Adriana Ant?nia da Cruz ; Capobianco, Marcela P ; Storti-Melo, Luciane Moreno ; Cunha, Maristela G ; Cassiano, Gustavo Capatti ; Machado, Ricardo Luiz Dantas
Data: 2017
Origem: Oasisbr
Assunto(s): Plasmodium vivax / imunologia; Mal?ria Vivax / imunologia; Imunoglobulina G / imunologia; Citocinas / gen?tica; Polimorfismo Gen?tico; Polimorfismo de Fragmento de Restri??o / gen?tica; Ensaio de Imunoadsor??o Enzim?tica / m?todos; Rea??o em Cadeia da Polimerase / m?todos; Ecossistema Amaz?nico (BR)
Descrição
This study was financed by the Brazilian National Council for Scientific and Technological Development (Conselho Nacional de Desenvolvimento Cient?? fico e Tecnol?gico) process number 472135/2012, the Par? State Research Support Foundation (Funda??o de Amparo ? Pesquisa do Estado do Par?) and CAPPES. We thank Dr. Irene Soares and Luzia Carvalho for their help in serological analysis. This project was funded by CNPq and Capes.
S?o Jos? do Rio Preto Medical School. Department of Dermatology, Infectious and Parasitic Diseases. S?o Jos? do Rio Preto, SP, Brazil.
S?o Paulo State University. Department of Biology. S?o Jos? do Rio Preto, SP, Brazil.
Federal University of Sergipe. Department of Biology. Laboratory of Molecular Genetics and Biotechnology. S?o Crist?v?o, SE, Brazil.
Federal University of Par?. Institute of Biological Sciences. Laboratory of Microbiology and Immunology. Bel?m, PA, Brazil.
Universidade de Campinas. Department of Genetics, Evolution and Bioagents. Laboratory of Tropical Diseases. Campinas, SP, Brazil.
S?o Jos? do Rio Preto Medical School. Department of Dermatology, Infectious and Parasitic Diseases. S?o Jos? do Rio Preto, SP, Brazil / S?o Paulo State University. Department of Biology. S?o Jos? do Rio Preto, SP, Brazil / Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Laborat?rio de Pesquisa B?sica em Mal?ria. Ananindeua, PA, Brasil.
The immune response against Plasmodium vivax immunogenic epitopes is regulated by pro- and anti-inflammatory cytokines that determine antibody levels and class switching. Cytokine gene polymorphisms may be responsible for changes in the humoral immune response against malaria. The aim of this study was to evaluate whether polymorphisms in the TNFA, IFNG and IL10 genes would alter the levels of anti-PvAMA1, PvDBP and -PvMSP119 IgG antibodies in patients with vivax malaria. Methods: Samples from 90 vivax malaria-infected and 51 uninfected subjects from an endemic area of the Brazilian Amazon were genotyped using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) to identify polymorphisms of the genes TNFA (-1031T > C, -308G > A, -238G > A), IFNG (+874T > A) and IL10 (-819C > T, -592C > A). The levels of total IgG against PvAMA1, PvDBP and PvMSP-119 were determined using an enzyme-linked immunosorbent assay (ELISA). Associations between the polymorphisms and the antibody response were assessed by means of logistic regression models. Results: No significant differences were found in the levels of IgG antibodies against the PvAMA-1, PvDBP or PvMSP119 proteins in relation to the studied polymorphisms. Conclusions: Although no associations were found among the evaluated genotypes and alleles and anti-merozoite IgG class P. vivax antibody levels, this study helps elucidate the immunogenic profile involved in the humoral immune response in malaria.
