Autor(es):
Concei??o, Emilyn Costa ; Refregier, Guislaine ; Gomes, Harrison Magdinier ; Olessa-Daragon, Xavier ; Coll, Francesc ; Ratovonirina, Noel Harijaona ; Rasolofo-Razanamparany, Voahangy ; Lopes, Maria Luiza ; Soolingen, Dick Van ; Rutaihwa, Liliana ; Gagneux, Sebastien ; Bollela, Valdes Roberto ; Suffys, Philip Noel ; Duarte, Rafael Silva ; Lima, Karla Val?ria Batista ; Sola, Christophe Noel
Data: 2019
Origem: Oasisbr
Assunto(s): Mycobacterium tuberculosis / isolamento & purifica??o; Mycobacterium tuberculosis / patogenicidade; Filogenia; Evolu??o Molecular; Polimorfismo de Nucleot?deo ?nico; Sequenciamento Completo do Genoma / m?todos; Linhagem
Descrição
Universit? Paris-Sud. Universit? Paris Saclay. Institut de Biologie Int?grative de la Cellule. I2BC. UMR9198. CEA. CNRS. Gif-sur-Yvette cedex, France / Universidade Federal do Rio de Janeiro. P?s-Gradua??o Instituto de Microbiologia Professor Paulo de G?es. Rio de Janeiro, RJ, Brazil.
Universit? Paris-Sud. Universit? Paris Saclay. Institut de Biologie Int?grative de la Cellule. I2BC. UMR9198. CEA. CNRS. Gif-sur-Yvette cedex, France.
Universit? Paris-Sud. Universit? Paris Saclay. Institut de Biologie Int?grative de la Cellule. I2BC. UMR9198. CEA. CNRS. Gif-sur-Yvette cedex, France / Funda??o Oswaldo Cruz. Instituto Oswaldo Cruz. Laborat?rio de Biologia Molecular Aplicada a Micobact?ria. Rio de Janeiro, RJ, Brazil.
Universit? Paris-Sud. Universit? Paris Saclay. Institut de Biologie Int?grative de la Cellule. I2BC. UMR9198. CEA. CNRS. Gif-sur-Yvette cedex, France.
London School of Hygiene & Tropical Medicine. Faculty of Infectious and Tropical Diseases. London, UK.
Universit? Paris-Sud. Universit? Paris Saclay. Institut de Biologie Int?grative de la Cellule. I2BC. UMR9198. CEA. CNRS. Gif-sur-Yvette cedex, France / Institut Pasteur de Madagascar. Unit? des Mycobact?ries. Antananarivo, Madagascar.
Institut Pasteur de Madagascar. Unit? des Mycobact?ries. Antananarivo, Madagascar.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
National Institute for Public Health and the Environment. Bilthoven, the Netherlands / Radboud University Nijmegen Medical Centre. Department of Medical Microbiology. Nijmegen, the Netherlands.
Swiss Tropical & Public Health Institute. Basel, Switzerland / University of Basel. Basel, Switzerland.
Swiss Tropical & Public Health Institute. Basel, Switzerland / University of Basel. Basel, Switzerland.
Universidade de S?o Paulo. Faculdade de Medicina de Ribeir?o Preto. Ribeir?o Preto, SP, Brazil.
Funda??o Oswaldo Cruz. Instituto Oswaldo Cruz. Laborat?rio de Biologia Molecular Aplicada a Micobact?ria. Rio de Janeiro, RJ, Brazil.
Universidade Federal do Rio de Janeiro. P?s-Gradua??o Instituto de Microbiologia Professor Paulo de G?es. Rio de Janeiro, RJ, Brazil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
Universit? Paris-Sud. Universit? Paris Saclay. Institut de Biologie Int?grative de la Cellule. I2BC. UMR9198. CEA. CNRS. Gif-sur-Yvette cedex, France / Universidade de S?o Paulo. Faculdade de Medicina de Ribeir?o Preto. Ribeir?o Preto, SP, Brazil.
Lineage 1 (L1) is one of seven Mycobacterium tuberculosis complex (MTBC) lineages. The objective of this study was to improve the complex taxonomy of L1 using phylogenetic SNPs, and to look for the origin of the main L1 sublineage prevalent in Para, Brazil. We developed a high-throughput SNPs-typing assay based on 12-L1-specific SNPs. This assay allowed us to experimentally retrieve SNP patterns on nine of these twelve SNPs in 277 isolates previously tentatively assigned to L1 spoligotyping-based sub lineages. Three collections were used: Par?-Brazil (71); RIVM, the Netherlands (102), Madagascar (104). One-hundred more results were generated in Silico using the PolyTB database. Based on the final SNPs combination, the samples were classified into 11 clusters (C1-C11). Most isolates within a SNP-based cluster shared a mutual spoligotyping-defined lineage. However, L1/EAI1-SOM (SIT48, sp. 40) and L1/EAI6-BGD1 (SIT591, sp. 23) showed a poor correlation with SNP data and are not monophyletic. L1/EAI8-MDG and L1/EAI3-IND belonged to C5; this result suggests that they share a common ancestor. L1.1.3/SIT129, a spoligotype pattern found in SNPs-cluster C6, was found to be shared between Par?/Brazil and Malawi. SIT129 was independently found to be highly prevalent in Mozambique, which suggests a migration history from East-Africa to Brazil during the 16th?18th slave trade period to Northern Brazil.
