Autor(es): Aguilar, Patricia V ; Souza, William Marciel de ; Silvas, Jesus A ; Wood, Thomas ; Widen, Steven ; Fumagalli, Marc?lio Jorge ; Nunes, Marcio Roberto Teixeira
Data: 2019
Origem: Oasisbr
Assunto(s): Arbovirus / classifica??o; Genoma / gen?tica; V?rus Estero Real; Ornithodoros / gen?tica; Filogenia / m?todos; Sancti Sp?ritus (CU)
F31 AI124662/AI/NIAID NIH HHS/United States R24 AI120942/AI/NIAID NIH HHS/United States
University of Texas Medical Branch. Department of Pathology. Galveston, Texas, USA / Institute for Human Infection and Immunity. Galveston, Texas, USA / Center for Tropical Diseases. Galveston, Texas, USA.
University of S?o Paulo. School of Medicine of Ribeir?o Preto. Virology Research Center. Ribeir?o Preto, SP, Brazil.
University of Texas Medical Branch. Department of Pathology. Galveston, Texas, USA / Institute for Human Infection and Immunity. Galveston, Texas, USA / Center for Tropical Diseases. Galveston, Texas, USA.
University of Texas Medical Branch. Department of Biochemistry and Molecular Biology. Galveston, Texas, USA.
University of Texas Medical Branch. Department of Biochemistry and Molecular Biology. Galveston, Texas, USA.
University of S?o Paulo. School of Medicine of Ribeir?o Preto. Virology Research Center. Ribeir?o Preto, SP, Brazil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
Estero Real virus (ERV) was isolated in 1980 from Ornithodoros tadaridae ticks collected in El Estero Real, Sancti Spiritus, Cuba. Antigenic characterization of the isolate based on serological methods found a relationship with Abras and Zegla viruses and, consequently, the virus was classified taxonomically within the Patois serogroup. Given the fact that genetic characterization of Patois serogroup viruses has not yet been reported and that ERV is the only virus within the Patois serogroup isolated from ticks, we recently conducted nearly complete genome sequencing in an attempt to gain further insight into the genetic relationship of ERV with other Patois serogroup viruses and members of Peribunyaviridae family (Bunyavirales order). With the exception of ERV, our sequencing and phylogenetic studies revealed the close relationship of the Patois serogroup viruses to each other, forming a clear divergent clade from other members of the Orthobunyavirus genus (Peribunyaviridae family). Notably, our analysis also revealed that ERV forms a monophyletic clade that is closely related to species of the Orthonairovirus genus (Nairoviridae family) in all the genome segments. In light of these findings, we believe that the taxonomic classification of ERV should be revised.
