Autor(es):
Costa, Fl?via P?voa da ; Puty, Bruna ; Nogueira, Lygia S ; Mitre, Geovanni Pereira ; Santos, S?vio Monteiro dos ; Teixeira, Bruno Jos? Brito ; Kataoka, Maria Sueli da Silva ; Martins, Manoela Domingues ; Barboza, Carlos Augusto Galv?o ; Monteiro, Marta Chagas ; Rogez, Herv? ; Oliveira, Edivaldo Herculano Corr?a de ; Lima, Rafael Rodrigues
Data: 2020
Origem: Oasisbr
Assunto(s): Doen?as Periodontais / patologia; Per?xido de Hidrog?nio / administra??o & dosagem; Ligamento Periodontal; Estresse Oxidativo / efeitos dos f?rmacos; Antioxidantes / farmacologia
Descrição
Funding: Amazon Foundation of Support for Studies and Research (FAPESPA). Brazilian Federal Agency for Support and Evaluation of Graduate Education (CAPES- finance code 001)
Federal University of Par?. Institute of Biological Sciences. Laboratory of Functional and Structural Biology. Bel?m, PA, Brazil / Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas.Laborat?rio de Cultura de Tecidos e Citogen?tica. Ananindeua, PA, Brasil.
Federal University of Par?. Institute of Biological Sciences. Laboratory of Functional and Structural Biology. Bel?m, PA, Brazil / Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas.Laborat?rio de Cultura de Tecidos e Citogen?tica. Ananindeua, PA, Brasil.
Federal University of Par?. Institute of Biological Sciences. Laboratory of Functional and Structural Biology. Bel?m, PA, Brazil / Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas.Laborat?rio de Cultura de Tecidos e Citogen?tica. Ananindeua, PA, Brasil.
Federal University of Par?. Faculty of Dentistry. Laboratory of Cell Culture. Bel?m, PA, Brazil.
Federal University of Par?. Faculty of Pharmacy. Laboratory of Oxidative Stress and Clinical Immunology. Bel?m, PA, Brazil.
Federal University of Par?. Center for Valorization of Amazonian Bioactive Compounds. Bel?m, PA, Brazil.
Federal University of Par?. Faculty of Dentistry. Laboratory of Cell Culture. Bel?m, PA, Brazil.
Federal University of Rio Grande do Sul. School of Dentistry. Department of Oral Pathology. Porto Alegre, RS, Brazil.
Federal University of Rio Grande do Norte. Department of Morphology. Natal, RN, Brazil.
Federal University of Par?. Faculty of Pharmacy. Laboratory of Oxidative Stress and Clinical Immunology. Bel?m, PA, Brazil.
Federal University of Par?. Center for Valorization of Amazonian Bioactive Compounds. Bel?m, PA, Brazil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas.Laborat?rio de Cultura de Tecidos e Citogen?tica. Ananindeua, PA, Brasil.
Federal University of Par?. Institute of Biological Sciences. Laboratory of Functional and Structural Biology. Bel?m, PA, Brazil.
Piceatannol is a resveratrol metabolite that is considered a potent antioxidant and cytoprotector because of its high capacity to chelate/sequester reactive oxygen species. In pathogenesis of periodontal diseases, the imbalance of reactive oxygen species is closely related to the disorder in the cells and may cause changes in cellular metabolism and mitochondrial activity, which is implicated in oxidative stress status or even in cell death. In this way, this study aimed to evaluate piceatannol as cytoprotector in culture of human periodontal ligament fibroblasts through in vitro analyses of cell viability and oxidative stress parameters after oxidative stress induced as an injury simulator. Fibroblasts were seeded and divided into the following study groups: control, vehicle, control piceatannol, H2O2 exposure, and H2O2 exposure combined with the maintenance in piceatannol ranging from 0.1 to 20 ?M. The parameters analyzed following exposure were cell viability by trypan blue exclusion test, general metabolism status by the 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) method, mitochondrial activity through the ATP production, total antioxidant capacity, and reduced gluthatione. Piceatannol was shown to be cytoprotective due the maintenance of cell viability between 1 and 10 ?M even in the presence of H2O2. In a concentration of 0.1 ?M piceatannol decreased significantly cell viability but increased cellular metabolism and antioxidant capacity of the fibroblasts. On the other hand, the fibroblasts treated with piceatannol at 1 ?M presented low metabolism and antioxidant capacity. However, piceatannol did not protect cells from mitochondrial damage as measured by ATP production. In summary, piceatannol is a potent antioxidant in low concentrations with cytoprotective capacity, but it does not prevent all damage caused by hydrogen peroxide
