Autor(es):
Capobianco, Marcela Petrolini ; Cassiano, Gustavo Capatti ; Storti-Melo, Luciane Moreno ; Pimenta, Tamirys Sim?o ; Rodrigues, Ana Paula Drummond ; Arruda, Jos? Eduardo Gomes ; Pinto, Marcia Ribeiro ; Baptista, Andrea Regina de Souza ; Pratt-Riccio, Lilian Rose ; Bonini-Domingos, Cl?udia Regina ; Oliveira-Ferreira, Joseli de ; Machado, Ricardo Luiz Dantas
Data: 2020
Origem: Oasisbr
Assunto(s): Plasmodium vivax / parasitologia; Polimorfismo Gen?tico; Citocinas; Prote?na Circunsporozo?ta; Anticorpos; Interleucina-1beta / gen?tica; Goian?sia do Par? (PA)
Descrição
Conselho Nacional para o Desenvolvimento Cient?fico e Tecnol?gico (CNPq) ; Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior, Brazil (CAPES ? DS and PDSE) ? Finance Code 001
S?o Paulo State University. Graduate Program in Biosciences. S?o Jos? do Rio Preto, SP, Brazil.
Lisboa University. Tropical Medicine and Hygiene Institut. Global Health and Tropical Medicine. Lisboa, Portugal.
Federal University of Sergipe. Department of Biology. Aracaj?, SE, Brazil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Laborat?rio de Imunogen?tica da Mal?ria Ananindeua, PA, Brasil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Laborat?rio de Microscopia Eletr?nica. Ananindeua, PA, Brasil / Universidade Federal do Par?. Bel?m, PA, Brasil.
Fluminense Federal University. Center of Microorganisms Investigation. Niter?i, RJ, Brazil.
Fluminense Federal University. Center of Microorganisms Investigation. Niter?i, RJ, Brazil.
Fluminense Federal University. Center of Microorganisms Investigation. Niter?i, RJ, Brazil.
Funda??o Oswaldo Cruz. Oswaldo Cruz Institute. Laboratory of Malaria Research. Rio de Janeiro, RJ, Brazil.
S?o Paulo State University. Graduate Program in Biosciences. S?o Jos? do Rio Preto, SP, Brazil.
Funda??o Oswaldo Cruz. Oswaldo Cruz Institute. Laboratory of Immunoparasitology. Rio de Janeiro, RJ, Brazil.
S?o Paulo State University. Graduate Program in Biosciences. S?o Jos? do Rio Preto, SP, Brazil / Fluminense Federal University. Center of Microorganisms Investigation. Niter?i, RJ, Brazil.
BACKGROUND: It is well established that infection by Plasmodium vivax is a result of host-parasite interactions. In the present study, association with the IL1/IL2 cytokine profiles, anticircumsporozoite protein antibody levels and parasitic loads was evaluated in individuals naturally infected with P. vivax in an endemic area of the Brazilian Amazon. METHODS: Molecular diagnosis of P. vivax and variants was performed using the PCR-RFLP method and IL1B -511C>T, IL2 -330T>G and IL2+114T>G polymorphisms were identified using PCR-RFLP and allele-specific PCR. IL-1? and IL-2 cytokine levels were detected by flow cytometry and circumsporozoite protein (CSP) antibodies were measured by ELISA. RESULTS: Three variants of P. vivax CSP were identified and VK247 was found to be the most frequent. However, the prevalence and magnitude of IgG antibodies were higher for the VK210 variant. Furthermore, the antibody response to the CSP variants was not associated with the presence of the variant in the infection. Significant differences were observed between the single nucleotide polymorphism (SNP) -511T>C in the IL1B gene and levels of antibodies to the VK247 and P. vivax-like variants, but there were no associations between SNPs in IL1 and IL2 genes and their plasma products. CONCLUSIONS: Individuals with the rs16944 CC genotype in the IL1? gene have higher antibody levels to the CSP of P. vivax of VK247 and P. vivax-like variants.
