Autor(es): Santos, Joselita Maria Mendes dos ; Tadei, Wanderli Pedro ; Contel, Eucléia Primo Betioli
Data: 2020
Origem: Oasisbr
Assunto(s): Cytosol Aminopeptidase; Esterases; Glycerol 3 Phosphate Dehydrogenase; Animals; Anopheles; Enzymology; Gene Expression; Genetics; Growth, Development And Aging; Metabolism; Animal; Anopheles; Esterases; Gene Expression; Glycerolphosphate Dehydrogenase; Leucyl Aminopeptidase
The esterases, leucine aminopeptidase and alpha-glycerophosphate dehydrogenase revealed modifications in gene expressions during the development of Anopheles darlingi. The esterases showed five activity bands, 1 and 2 being more deeply stained during the larval stages than in pupae or adults, esterases 3 and 4 more deeply stained in pupae and adults whereas esterase 5 was present throughout development. Leucine aminopeptidase showed five activity bands: LAP2 and LAP5 were characteristic of larvae, LAP3 was specific for pupae and adults, LAP4 was detected only in pupae, and LAP1 and LAP6 were detected in all stages. alpha-Glycerophosphate dehydrogenase presented one activity band on starch gel whose intensity increased with development. Two activity bands were detected on polyacrylamide gel (alpha-GPDH1 and alpha-GPDH2) in 4th-instar larvae (old pigmented larvae) and this activity increased with development.
