A Standardized Approach for Multispecies Purification of Mammalian Male Germ Cells by Mechanical Tissue Dissociation and Flow Cytometry

• Author(s): Lima, AC ; Jung, M ; Rusch, J ; Usmani, A ; Lopes, AM ; Conrad, DF
• Date: 2017
• Persistent ID: http://hdl.handle.net/10216/114551
• Origin: Repositório Aberto da Universidade do Porto
• Subject(s): Animals; Flow Cytometry/methods; Germ Cells/cytology; Germ Cells/metabolism| | Male; Mice; Spermatogenesis/physiology; Spermatogonia/cytology; Testis/cytology; Testis/metabolism
• Document Type: Journal article
• Language: English

Description

Fluorescence-activated cell sorting (FACS) has been one of the methods of choice to isolate enriched populations of mammalian testicular germ cells. Currently, it allows the discrimination of up to 9 murine germ cell populations with high yield and purity