Author(s): Domingos, J ; Casimiro, A ; Portugal-Calisto, D ; Varandas, L ; Nogueira, F ; Silva, M S
Date: 2018
Persistent ID: http://hdl.handle.net/10400.17/2965
Origin: Repositório do Centro Hospitalar de Lisboa Central, EPE
Subject(s): P. Falciparum antibodies; Plasmodium Falciparum; Humoral Immunity; malaria antigens; Severe Malaria; HDE INF PED
Malaria is a parasitic disease of which Plasmodium falciparum causes the most severe form of the disease. The immune response against Plasmodium spp. is complex and remains unclear. The present report aimed to better understand the humoral immune response in severe malaria and analyse new immunodominant antigen candidates as possible serological marker in severe malaria in children. This study included children aged 0-16 years from Guinea-Bissau with clinical signs of severe malaria. Serological and immunochemical characterisation of different anti-P. falciparum antibodies were made by ELISA and immunoblotting using a crude protein extract of P. falciparum. Sera from 12 children with severe malaria were analysed. Nine samples were positive for total anti-P. falciparum antibodies, seven for IgM and eight for total IgG anti-P. falciparum. There was a predominance of IgG1 response, suggesting a cytophilic action in severe malaria and a major role of IgG1 over other immunoglobulins. The antigenic profile of P. falciparum showed a consistent immunoblotting pattern of approximately 180 kDa, 100 kDa and around 50-40 kDa. The serological reactivity found in protein bands makes them as immunodominant antigens and promising candidates for serological markers in the context of severe malaria.
