Author(s): Ayhan, Nazli ; Baklouti, Amal ; Prudhomme, Jorian ; Walder, Gernot ; Amaro, Fatima ; Alten, Bulent ; Moutailler, Sara ; Ergunay, Koray ; Charrel, Remi N ; Huemer, Hartwig
Date: 2017
Persistent ID: http://hdl.handle.net/10400.18/5455
Origin: Repositório Científico do Instituto Nacional de Saúde
Subject(s): Animal Distribution; Animals; Insect Vectors; Phlebovirus; Phylogeny; Psychodidae; Research Design; Infecções Sistémicas e Zoonoses
The purpose of this review is to provide practical information to help researchers intending to perform "from field to laboratory" studies on phleboviruses transmitted by sandflies. This guideline addresses the different steps to be considered starting from the field collection of sandflies to the laboratory techniques aiming at the detection, isolation, and characterization of sandfly-borne phleboviruses. In this guideline article, we address the impact of various types of data for an optimal organization of the field work intending to collect wildlife sandflies for subsequent virology studies. Analysis of different data sets should result in the geographic positioning of the trapping stations. The overall planning, the equipment and tools needed, the manpower to be deployed, and the logistics to be anticipated and set up should be organized according to the objectives of the field study for optimal efficiency.
