Autor(es): Martingo, Maria ; Baptista-Silva, Sara ; Mesquita, Raquel ; Ferreira, João Paulo ; Borges, Sandra ; Pintado, Manuela
Data: 2025
Identificador Persistente: http://hdl.handle.net/10400.14/52984
Origem: Veritati - Repositório Institucional da Universidade Católica Portuguesa
Assunto(s): Bio-based membrane; Chitosan; Hemodialysis treatment; Tenebrio molitor
This study introduces a sustainable and efficient alternative to traditional chitosan sources derived from crustaceans, exploring the extraction and application of insect-derived chitosan from Tenebrio molitor for hemodialysis (HD) membranes design. Efficient extraction and deacetylation methods were tested and developed between 6 h and 12 h to obtain chitosan. Chitin was isolated from T. molitor through deproteinization and demineralization, with yields of approximately 5 % (w/w). Chitosan was obtained from the extracted chitin resulting in yields of between 65.0 and 79.3 (w/w). Characterization using FTIR confirmed structural similarities with commercial chitosan and degrees of deacetylation in the 73–75 % range. The bioactive properties of chitosan obtained from T. molitor, including antimicrobial and antioxidant activities, were evaluated. All the microorganisms tested were inhibited, exhibiting minimum lethal concentrations between 2 and 8 mg/mL. In addition, chitosan showed antioxidant activity in the range of 60–65 μmol Trolox equivalent/g, suggesting its viability for various medical applications. This study additionally allowed the design of sustainable hybrid chitosan membrane (CH-M) tailored for HD applications. The permeation characteristics of CH-M for urea and albumin were studied in vitro to assess their suitability as HD membranes. Urea was permeable to values of over 70 % and albumin was retained. Also, cytotoxicity assays against L929 fibroblast cells demonstrated that the CH-M samples exhibit low metabolic inhibition (around 15 %) The application of CH-M in HD represents a significant advance, offering the potential for enhanced therapeutic outcomes for chronic kidney disease (CKD).
