Autor(es): Prieto-Fernández, Ángeles ; Pereira, Sofia ; Sousa, Ana Sofia ; Rodríguez-Garrido, Beatriz ; González-Prieto, Serafin ; Castro, Paula ; Trasar-Cepeda, Carmen
Data: 2025
Identificador Persistente: http://hdl.handle.net/10400.14/54716
Origem: Veritati - Repositório Institucional da Universidade Católica Portuguesa
Introduction: Sustainable agriculture increasingly relies on the use of environmentally friendly practices and biotechnological tools to maintain soil health and productivity. The ReCROP project, funded by the PRIMA-Med programme, aims to enhance soil functions and resilience through integrated cropping systems. As part of this initiative, we evaluated the effects of a plant growth-promoting (PGP) bacterial bioinoculant applied in a vineyard of the Mencía grape variety located in the Ribeiro Denomination of Origin (Galicia, NW Spain). This study explores the bioinoculant's influence on soil biochemical properties and must composition over two growing seasons. Experimental field site: The field trial was conducted in a vineyard located in the Denomination of Origin (D.O.) Ribeiro area, specifically in A Gandarela – Esposende, within the municipality of Ribadavia (Galicia, NW Spain). The vineyard is situated at GPS coordinates 42.2959, -8.1327 and is registered in the SIGPAC system under code 32 70 00 44 148. The site is representative of typical Ribeiro vineyards, characterized by a temperate Atlantic climate. The experimental area covered approximately 0.03 ha and consisted of three plots, each with an approximate area of 100 m². Material and methods: The study was conducted over two consecutive cropping seasons (2023 and 2024) in the described experimental vineyard. A plant growth-promoting (PGP) bacterial strain, Arthrobacter nicotinovorans EAPAA, was used as a bioinoculant. The selected PGP strain presents beneficial traits such as phosphorus solubilization and nitrogen fixation. The strain was cultured in Tryptic Soy Broth (TSB) and the inoculum consisted of 500 mL of a bacterial suspension with an optical density of 1.0, applied at the base of each grapevine stock in early spring. In each plot, four grapevines were inoculated and four were treated with 1/3 diluted TSB as controls. At harvest, soil and grape samples were collected from both non-inoculated and inoculated vines. Soil samples were taken at the base of each vine and pooled to obtain one composite sample for the inoculated and one for the non-inoculated treatments per plot. Conclusions: The bioinoculation with the PGP strain Arthrobacter nicotinovorans EAPAA in a Mencía vineyard led to several soil beneficial effects: Improved availability of labile C and labile P; Enhanced soil biological and biochemical activity, particularly of the N and P cycles. The bioinoculation also appears to induce modifications of must composition, which should be further studied to analyse their impact in the vinification process. These findings suggest that the bioinoculant can contribute to improving soil functionality and may influence grape quality, offering a promising strategy for sustainable vineyard management.
