Author(s):
Barral-Netto, Manoel ; Sohsten, R. L. Von ; Teixeira, M. ; Santos, Washington Luis Conrado dos ; Pompeu, M. L. ; Moreira, R. A. ; Oliveira, J. T. A. ; Cavada, B. S. ; Falcoff, E. ; Barral, Aldina Maria Prado ; Barral-Netto, Manoel ; Sohsten, R. L. Von ; Teixeira, M. ; Santos, Washington Luis Conrado dos ; Pompeu, M. L. ; Moreira, R. A. ; Oliveira, J. T. A. ; Cavada, B. S. ; Falcoff, E. ; Barral, Aldina Maria Prado
Date: 2013
Origin: Oasisbr
Subject(s): Leishmania amazonensis; Leishmaniasis; Leishmania; Tegumentary leishmaniasis; Experimental leishmaniasis; Canavalia brasiliensis; Interferon-γ
Description
Texto completo. Acesso restrito. p. 237–250
Submitted by Santiago Fabio (fabio.ssantiago@hotmail.com) on 2013-09-09T20:25:01Z No. of bitstreams: 1 66666666666666666.pdf: 979575 bytes, checksum: dfb8ac628ceaf8c94cb72e73fbaa01d7 (MD5)
Made available in DSpace on 2013-09-09T20:25:02Z (GMT). No. of bitstreams: 1 66666666666666666.pdf: 979575 bytes, checksum: dfb8ac628ceaf8c94cb72e73fbaa01d7 (MD5) Previous issue date: 1996
In vivo administration of Canavalia brasiliensis lectin (at the time of infection, or maintained throughout the infection) reduced the lesions of highly susceptible BALB/c mice infected by Leishmania amazonensis. At the doses used C. brasiliensis lectin (ConBr) does not interfere with penetration or fate of Leishmania in the macrophages in vitro. Since Interferon-γ (IFN-γ) is the major macrophage activating factor, and considered a critical element in the successful immune response against leishmaniasis, we explored its participation in this phenomenon. ConBr either in vivo or in vitro induced IFN-γ production in normal or in leishmania-infected BALB/c mice. However we were unable to change the course of disease by in vivo IFN-γ administration (although IFN-γ preparations were effective in inducing a leishmanicidal effect in vitro on L. amazonensis-infected peritoneal macrophages). Additionally, IFN-γ neutralization with anti-IFN-γ monoclonal antibody did not alter the protection conferred by ConBr administration. These data show that lectin administration in vivo is protective in the otherwise unchecked L. amazonensis infection of BALB/c mice, and suggest that such effect is not mediated by IFN-γ.
Salvador
