Author(s): Almeida, Cláudia Maria Costa de ; Flores, Victor Martin Quintana ; Medina-Acosta, Enrique ; Schriefer, Nicolaus Albert Borges ; Barral-Netto, Manoel ; Silva, Wilmar Dias da ; Almeida, Cláudia Maria Costa de ; Flores, Victor Martin Quintana ; Medina-Acosta, Enrique ; Schriefer, Nicolaus Albert Borges ; Barral-Netto, Manoel ; Silva, Wilmar Dias da
Date: 2014
Origin: Oasisbr
Texto completo: acesso restrito. p. 573–582
Submitted by Edileide Reis (leyde-landy@hotmail.com) on 2013-12-18T11:28:32Z No. of bitstreams: 1 C. M. C. De Almeida.pdf: 2417376 bytes, checksum: a9ce3051d1d24da394ead50296708427 (MD5)
Approved for entry into archive by Alda Lima da Silva (sivalda@ufba.br) on 2014-02-13T18:35:59Z (GMT) No. of bitstreams: 1 C. M. C. De Almeida.pdf: 2417376 bytes, checksum: a9ce3051d1d24da394ead50296708427 (MD5)
Made available in DSpace on 2014-02-13T18:35:59Z (GMT). No. of bitstreams: 1 C. M. C. De Almeida.pdf: 2417376 bytes, checksum: a9ce3051d1d24da394ead50296708427 (MD5) Previous issue date: 2003
Enteropathogenic Escherichia coli (EPEC) is a major aetiological agent of childhood diarrhoea in developing countries. The structural repeating protein A subunit, BfpA, found in the bundle-forming pilus, is one of the virulent factors for EPEC pathogenesis. Recombinant BfpA in laying hens elicited sustained and vigorous antibody production. Immunoglobulin Y (IgY) anti-BfpA antibodies were recovered from egg yolk, purified and characterized. Immunoadsorption with whole extracts of the isogenic E. coli EPEC adherence factor (EAF) strain that lacks BfpA rendered the resulting IgY preparations capable of: (a) recognizing purified or recombinant BfpA proteins in a dose-dependent fashion; (b) blocking the colonization of HeLa cells by EPEC EAF+, in vitro; (c) specifically identifying E. coli bearing EAF+; and (d) inhibiting the growth of E. coli EAF+ but not the EAF strain. IgY anti-BfpA is potentially useful as a specific, low-cost immunobiological reagent to screen human faecal specimens for the presence of EPEC.
