Author(s): Ferreira, Joana ; Karolina Tkacz, Karolina ; Turkiewicz, Igor Piotr ; Santos, Isabel ; Silva, Mariana Camoesas e ; Lima, Ana ; Sousa, Isabel
Date: 2024
Persistent ID: http://hdl.handle.net/10400.5/102662
Origin: Repositório da Universidade de Lisboa
Subject(s): cholinesterase; MMP-9; phenolics; tocols; carotenoids; xanthophylls; procyanidins; aminoacids; AAT; chromatography
The fruit juice industry generates a significant amount of waste, with a strong impact on the environment and the economy. Therefore, researchers have been focusing on the characterization of resources considered as food waste. This work provides information about the lipophilic and polar metabolites of pear pomace flours (PPFs) as a tool that can shed more light on the bioactive potential of this residue. Using UPLC-PDA, UPLC-FLR, and GC-MS, the study identified and quantified PPF’s polar and non-polar metabolites. Essential, conditional, and non-essential amino acids were found, with asparagine being the most abundant. Isoprenoids, including lutein, zeaxanthin, and carotene isomers, ranged from 10.8 to 22.9 mg/100 g dw. Total flavonoids and phenolic compounds were 520.5–636.4 mg/100 g dw and 536.9–660.1 mg/100 g dw, respectively. Tocotrienols and to- copherols were identified, with concentrations of 173.1–347.0 mg/100 g dw and 468.7–913.4 mg/ 100 g dw . Fatty acids were the major non-polar compounds. All fractions significantly reduced matrix metalloproteinase-9 (MMP-9) activity. Although PPF had lower antioxidant potential (3–6 mmol Trolox/100 g dw), it inhibited AChE and BuChE by 23–30% compared to physostigmine salicylate. These findings suggest that pear pomace waste can be repurposed into functional products with valuable bioactive properties by re-introducing it in the food chain.
