16S Genomics for Diagnosing Invasive Bacterial Infection

• Author(s): Vale, Filipa ; Tanoeiro, Luis ; Marques, Andreia T.
• Date: 2022
• Persistent ID: http://hdl.handle.net/10451/52330
• Origin: Repositório da Universidade de Lisboa
• Subject(s): 16S rRNA; Bacteria identification; Biomarker; Blood culture; Invasive bacterial infection; Metagenomics; Microbiology; mNGS; Molecular diagnostic; Next-generation sequencing; PCR; PNA-FISH; Primers; qPCR; Sepsis
• Document Type: Book part
• Language: English
• Contributor(s): Repositório Científico de Acesso Aberto da ULisboa

Description

Invasive bacterial infections (IBI) are leading causes of global morbidity and mortality. Successful treatment of the patients depends on a rapid and accurate diagnosis. The current golden standard procedure for bacterial identification in clinical context requires bacterial culture and biochemical analysis—a slow, culture-dependent and possible subjective approach, presenting several limitations. Bacteria identification based on 16S rRNA gene represents a faster, culture-independent and objective alternative for diagnostic. Here, we give a general overview of 16S rRNA genomics as diagnosing tool for IBI, briefly presenting and discussing the most significant technical approaches for 16S rRNA-mediated diagnosis.