Autor(es): Soares, Vítor Yamashiro Rocha ; Silva, Jailthon Carlos Da ; Silva, Kleverton Ribeiro Da ; Cruz, Maria Do Socorro Pires E ; Santos, Marcos Pérsio Dantas ; Ribolla, Paulo Eduardo Martins ; Alonso, Diego Peres ; Coelho, Luiz Felipe Leomil ; Costa, Dorcas Lamounier ; Costa, Carlos Henrique Nery
Data: 2014
Identificador Persistente: http://hdl.handle.net/11449/109954
Origem: Oasisbr
Assunto(s): blood meal analysis; cytochrome B; PCR-RFLP
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An analysis of the dietary content of haematophagous insects can provide important information about the transmission networks of certain zoonoses. The present study evaluated the potential of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of the mitochondrial cytochrome B (cytb) gene to differentiate between vertebrate species that were identified as possible sources of sandfly meals. The complete cytb gene sequences of 11 vertebrate species available in the National Center for Biotechnology Information database were digested with Aci I, Alu I, Hae III and Rsa I restriction enzymes in silico using Restriction Mapper software. The cytb gene fragment (358 bp) was amplified from tissue samples of vertebrate species and the dietary contents of sandflies and digested with restriction enzymes. Vertebrate species presented a restriction fragment profile that differed from that of other species, with the exception of Canis familiaris and Cerdocyon thous. The 358 bp fragment was identified in 76 sandflies. Of these, 10 were evaluated using the restriction enzymes and the food sources were predicted for four: Homo sapiens (1), Bos taurus (1) and Equus caballus (2). Thus, the PCR-RFLP technique could be a potential method for identifying the food sources of arthropods. However, some points must be clarified regarding the applicability of the method, such as the extent of DNA degradation through intestinal digestion, the potential for multiple sources of blood meals and the need for greater knowledge regarding intraspecific variations in mtDNA.
Universidade Federal do Piauí Instituto de Doenças Tropicais Natan Portela Departamento de Medicina Comunitária
Universidade Federal do Piauí Departamento de Morfofisiologia Veterinária Laboratório de Sanidade Animal
Universidade Federal do Pará Instituto de Ciências Biológicas
Universidade Estadual Paulista Instituto de Biociências de Botucatu Departamento de Parasitologia
Universidade Federal de Alfenas Instituto de Ciências Biomédicas
Universidade Estadual Paulista Instituto de Biociências de Botucatu Departamento de Parasitologia
