Author(s):
Aguiar, Bruno Guedes Alcoforado ; Coelho, Daniela Lemos ; Costa, Dorcas Lamounier ; Drumond, Betânia Paiva ; Coelho, Luiz Felipe Leomil ; Figueiredo, Lívio Carvalho ; Zacarias, Danielle Alves ; Silva, Jailthon Carlos Da ; Alonso, Diego Peres [UNESP] ; Ribolla, Paulo Eduardo Martins ; Ishikawa, Edna Aoba Yassui ; Gaído, Samara Belchior ; Costa, Carlos Henrique Nery
Date: 2015
Persistent ID: http://hdl.handle.net/11449/114114
Origin: Oasisbr
Subject(s): Genetic diversity; Kala-azar; Visceral leishmaniasis; Macrophage inhibition factor; Tropical diseases; Single nucleotide polymorphism
Description
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Introduction Kala-azar is a disease resulting from infection by Leishmania donovani and Leishmania infantum. Most patients with the disease exhibit prolonged fever, wasting, anemia and hepatosplenomegaly without complications. However, some patients develop severe disease with hemorrhagic manifestations, bacterial infections, jaundice, and edema dyspnea, among other symptoms, followed by death. Among the parasite molecules that might influence the disease severity are the macrophage migration inhibitory factor-like proteins (MIF1 and MIF2) and N-acetylglucosamine-1-phosphotransferase (NAGT), which act in the first step of protein N-glycosylation. This study aimed to determine whether MIF1, MIF2 and NAGT are virulence factors for severe kala-azar. Methods To determine the parasite genotype in kala-azar patients from Northeastern Brazil, we sequenced the NAGT genes of L. infantum from 68 patients as well as the MIF1 and MIF2 genes from 76 different subjects with diverse clinical manifestations. After polymerase chain reaction (PCR), the fragments were sequenced, followed by polymorphism identification. Results The nucleotide sequencing of the 144 amplicons revealed the absence of genetic variability of the NAGT, MIF1 and MIF2 genes between the isolates. The conservation of these genes suggests that the clinical variability of kala-azar does not depend upon these genes. Additionally, this conservation suggests that these genes may be critical for parasite survival. Conclusions NAGT, MIF1 and MIF2 do not alter the severity of kala-azar. NAGT, MIF1 and MIF2 are highly conserved among different isolates of identical species and exhibit potential for use in phylogenetic inferences or molecular diagnosis.
Universidade Federal do Piauí Instituto de Doenças Tropicais Natan Portella Laboratório de Leishmanioses
Universidade Federal de Juiz de Fora Laboratório de Virologia
Universidade Federal de Alfenas Laboratório de Vacinas
Universidade Federal da Paraíba Sociais e Agrárias Centro de Ciências Humanas
Universidade Estadual Paulista Instituto de Biociências Departamento de Parasitologia
Universidade Federal do Pará Núcleo de Medicina Tropical Laboratório de Biologia Molecular
Universidade Estadual Paulista Instituto de Biociências Departamento de Parasitologia
