Autor(es): Kopacz, Malgorzata M. ; Lorenzoni, André S. G. ; Polaquini, Carlos R. [UNESP] ; Regasini, Luis O. [UNESP] ; Scheffers, Dirk-Jan
Data: 2018
Identificador Persistente: http://hdl.handle.net/11449/171396
Origem: Oasisbr
Assunto(s): antibacterials; cell division; FtsZ; gallates; gallic acid; GTPase; membrane permeabilization
Made available in DSpace on 2018-12-11T16:55:10Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-01-01
Xanthomonas citri subsp. citri (Xac) is the causative agent of citrus canker, a plant disease that significantly impacts citriculture. In earlier work, we showed that alkylated derivatives of gallic acid have antibacterial action against Xac and target both the cell division protein FtsZ and membrane integrity in Bacillus subtilis. Here, we have purified native XacFtsZ and characterized its GTP hydrolysis and polymerization properties. In a surprising manner, inhibition of XacFtsZ activity by alkyl gallates is not as strong as observed earlier with B. subtilis FtsZ. As the alkyl gallates efficiently permeabilize Xac membranes, we propose that this is the primary mode of antibacterial action of these compounds.
Department of Molecular Microbiology Groningen Biomolecular Sciences and Biotechnology Institute University of Groningen
Laboratory of Antibiotics and Chemotherapeutics Department of Chemistry and Environmental Sciences Institute of Biosciences Humanities and Exact Sciences São Paulo State University (UNESP)
Department of Chemical Engineering Biotechnology and Environmental Technology University of Southern Denmark
Laboratory of Antibiotics and Chemotherapeutics Department of Chemistry and Environmental Sciences Institute of Biosciences Humanities and Exact Sciences São Paulo State University (UNESP)
