Autor(es):
Marcos, Caroline Maria [UNESP] ; da Silva, Julhiany de Fátima [UNESP] ; de Oliveira, Haroldo Cesar [UNESP] ; Assato, Patrícia Akemi [UNESP] ; Singulani, Junya de Lacorte [UNESP] ; Lopez, Angela Maria ; Tamayo, Diana Patricia ; Hernandez-Ruiz, Orville ; McEwen, Juan G. ; Mendes-Giannini, Maria José Soares [UNESP] ; Fusco-Almeida, Ana Marisa [UNESP]
Data: 2018
Identificador Persistente: http://hdl.handle.net/11449/177894
Origem: Oasisbr
Assunto(s): 14-3-3 protein; gene knockdown; morphological alterations; Paracoccidioides brasiliensis; virulence
Descrição
Made available in DSpace on 2018-12-11T17:27:36Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-02-17
The interaction between the fungal pathogen Paracoccidioides brasiliensis and host cells is usually mediated by specific binding events between adhesins on the fungal surface and receptors on the host extracellular matrix or cell surface. One molecule implicated in the P. brasiliensis-host interaction is the 14-3-3 protein. The 14-3-3 protein belongs to a family of conserved regulatory molecules that are expressed in all eukaryotic cells and are involved in diverse cellular functions. Here, we investigated the relevance of the 14-3-3 protein to the virulence of P. brasiliensis. Using antisense RNA technology and Agrobacterium tumefaciens-mediated transformation, we generated a 14-3-3-silenced strain (expression reduced by ˜55%). This strain allowed us to investigate the interaction between 14-3-3 and the host and to correlate the functions of P. brasiliensis 14-3-3 with cellular features, such as morphological characteristics and virulence, that are important for pathogenesis.
UNESP - Univ Estadual Paulista Departamento de Análises Clínicas; Laboratório de Micologia Clínica
Unidad de Biología Celular y Molecular Corporación para Investigaciones Biológicas (CIB) - Medellín
Escuela de Microbiología Universidad de Antioquia
Universidad de Antioquia
UNESP - Univ Estadual Paulista Departamento de Análises Clínicas; Laboratório de Micologia Clínica
