Autor(es): Venturini, James [UNESP] ; Fraga-Silva, Thais Fernanda Campos [UNESP] ; Marchetti, Camila Martins [UNESP] ; Mimura, Luiza Ayumi Nishiyama [UNESP] ; Conti, Bruno José [UNESP] ; Golim, Márjorie de Assis [UNESP] ; Mendes, Rinaldo Poncio [UNESP] ; de Arruda, Maria Sueli Parreira [UNESP]
Data: 2018
Identificador Persistente: http://hdl.handle.net/11449/177980
Origem: Oasisbr
Assunto(s): Alloxan; candidiasis; dendritic cells; experimental diabetes mellitus; macrophage activity
Made available in DSpace on 2018-12-11T17:28:02Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-07-03
Bloodstream infections caused by Candida species are responsible for high morbidity and mortality, and diabetes mellitus (DM) is an important underlying disease in candidemia episodes. Although DM patients show an enhanced proinflammatory profile, they are highly susceptible to mycobacterial and mycotic infections. Attempting to understand this paradox, we investigated if imbalanced macrophage and dendritic cell (DC) activations could be associated to high incidence and/or severity of Candida albicans infection in the hypoinsulinemia-hyperglycemia (HH) milieu. HH alloxan-induced mice were infected with C. albicans and peritoneal aderent phagocytes were co-cultured with or without lipopolyssaccharide or heat-killed C. albicans, and the production of cytotoxic metabolites, cytokines, and chemokines was evaluated. We also evaluated the surface expression of MHC-II and CD86 in splenic DCs. Our findings showed that both uninfected and C. albicans-infected HH mice showed less production of CCL2 and reduced expression of CD86 by peritoneal phagocytes and splenic DCs, respectively.
Faculdade de Ciências UNESP – Universidade Estadual Paulista
Instituto de Biocências de Botucatu Universidade Estadual Paulista
Faculdade de Medicina de Botucatu UNESP – Universidade Estadual Paulista
Faculdade de Ciências UNESP – Universidade Estadual Paulista
Instituto de Biocências de Botucatu Universidade Estadual Paulista
Faculdade de Medicina de Botucatu UNESP – Universidade Estadual Paulista
