Autor(es): Oliveira, Emerson A. ; Martins, Euder G. A. ; Soares, Marisi G. ; Chagas-Paula, Daniela A. ; Passero, Luiz F. D. [UNESP] ; Sartorelli, Patricia ; Baldim, Joao L. ; Lago, Joao Henrique G.
Data: 2019
Identificador Persistente: http://hdl.handle.net/11449/185843
Origem: Oasisbr
Assunto(s): Guarea macrophylla; 1,10-di-epi-cubenol; multivariate statistical analysis; antileishmanial activity
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Meliaceae representatives are economically important in several aspects including the production of highly prized woods (mahogany, cedar, etc.), constituents for cosmetics, and insecticides. The present study aimed to verify the chemical composition as well as leishmanicidal and cytotoxic potential of essential oils from leaves of two different populations of Guarea macrophylla collected at cities of Sao Paulo (population I) and Cubatao (population II), Sao Paulo State, Brazil. Chemically, the oils showed the predominance of sesquiterpenes: cis-beta-guaiene, bicyclogermacrene, viridiflorol, and isolongifolan-7 alpha-ol from population I and alpha-copaene, E-caryophyllene, cis-beta-guaiene, and gamma-amorphene from population II. In vitro antileishmanial activity against promastigote forms of Leishmania (L.) amazonensis of essential oils was evaluated and displayed 50% effective concentration (EC50) values ranging from 11.8 to 20.5 mu g mL(-1). Furthermore, toxicity against peritoneal macrophages of BALB/c mice was observed, with 50% cytotoxic concentration (CC50) ranging from 17.7 to > 100 mu g mL(-1). Multivariate statistical analysis revealed the influence of each constituent of the oils against L. amazonensis being 1,10-di-epi-cubenol, alpha-amorphene, E-caryophyllene, isopimara-7,15-diene, and beta-elemene associated with the antileishmanial potential.
Univ Fed Sao Paulo, Inst Ciencias Ambientais Quim & Farmaceut, BR-09972270 Diadema, SP, Brazil
Univ Sao Paulo, Inst Biociencias, BR-05508090 Sao Paulo, SP, Brazil
Univ Fed Alfenas, Inst Quim, BR-37130001 Alfenas, MG, Brazil
Univ Estadual Paulista, Inst Biociencias, BR-11330900 Sao Vicente, SP, Brazil
Univ Estadual Paulista, Inst Estudos Avancados Mar, BR-11330900 Sao Vicente, SP, Brazil
Univ Fed ABC, Ctr Ciencias Nat & Humanas, BR-09210580 Santo Andre, SP, Brazil
Univ Estadual Paulista, Inst Biociencias, BR-11330900 Sao Vicente, SP, Brazil
Univ Estadual Paulista, Inst Estudos Avancados Mar, BR-11330900 Sao Vicente, SP, Brazil
FAPESP: 2018/07885-1
FAPESP: 2016/00468-0
FAPESP: 2016/19269-8
CAPES: 001
