Author(s): Almeida, Lauana Fogaça de [UNESP] ; Moraes, Leonardo Nazário de [UNESP] ; Santos, Lucilene Delazari dos [UNESP] ; Valente, Guilherme Targino [UNESP]
Date: 2019
Persistent ID: http://hdl.handle.net/11449/188534
Origin: Oasisbr
Subject(s): Protein extraction protocol; Proteomics; Protocol comparisons; Saccharomyces cerevisiae proteins
Made available in DSpace on 2019-10-06T16:11:18Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-02-15
Universidade Estadual Paulista
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Mass spectrometry is the most used method for protein identification and quantification. Here we developed four protein extraction protocols precisely for mass spectrometry, and we compared with other ones already published. The best protocol developed by us consists on a simple extraction solution, a heat-shock step, and does not use protease inhibitor; moreover, it is the most efficient and uniform among replicates, besides to be safe, cheap and fast. That method also provided the highest number of proteins uniquely identified and allows finding a diversity of protein classes, which their absence is a problem to be avoided.
Sao Paulo State University (UNESP) Department of Bioprocess and Biotechnology School of Agriculture, Avenue Universitária, n 3780, Altos do Paraíso
Sao Paulo State University (UNESP) Center for the Study of Venoms and Venomous Animals (CEVAP), Avenue Universitária, n 3780, Altos do Paraíso
São Paulo State University (UNESP) Graduate Program in Tropical Diseases, Botucatu Medical School (FMB). Avenue Prof. Mário Rubens Guimarães Montenegro, no number, Rubião Jr
Sao Paulo State University (UNESP) Department of Bioprocess and Biotechnology School of Agriculture, Avenue Universitária, n 3780, Altos do Paraíso
Sao Paulo State University (UNESP) Center for the Study of Venoms and Venomous Animals (CEVAP), Avenue Universitária, n 3780, Altos do Paraíso
São Paulo State University (UNESP) Graduate Program in Tropical Diseases, Botucatu Medical School (FMB). Avenue Prof. Mário Rubens Guimarães Montenegro, no number, Rubião Jr
Universidade Estadual Paulista: 12/2015-PROPe
FAPESP: 2015/12093-9
