Author(s): Murata, Ramiro M. ; Branco-de-Almeida, Luciana S. ; Franco, Eliane M. ; Yatsuda, Regiane ; Santos, Marcelo H. dos ; Alencar, Severino M. de [UNESP] ; Koo, Hyun ; Rosalen, Pedro L.
Date: 2020
Persistent ID: http://hdl.handle.net/11449/194608
Origin: Oasisbr
Subject(s): benzophenone; Streptococcus mutans; dental caries; biofilms; fluoride; extracellular polysaccharides
Made available in DSpace on 2020-12-10T16:31:36Z (GMT). No. of bitstreams: 0 Previous issue date: 2010-01-01
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
USPHS Research grant from the National Institute of Dental and Craniofacial Research (National Institutes of Health, USA)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
7-Epiclusianone (7-epi), a novel naturally occurring compound isolated from Rheedia brasiliensis, effectively inhibits the synthesis of exopolymers and biofilm formation by Streptococcus mutans. In the present study, the ability of 7-epi, alone or in combination with fluoride (F), to disrupt biofilm development and pathogenicity of S. mutans in vivo was examined using a rodent model of dental caries. Treatment (twice-daily, 60s exposure) with 7-epi, alone or in combination with 125 ppm F, resulted in biofilms with less biomass and fewer insoluble glucans than did those treated with vehicle-control, and they also displayed significant cariostatic effects in vivo (p < 0.05). The combination 7-epi + 125 ppm F was as effective as 250 ppm F (positive-control) in reducing the development of both smooth-and sulcal-caries. No histopathological alterations were observed in the animals after the experimental period. The data show that 7-epiclusianone is a novel and effective antibiofilm/anticaries agent, which may enhance the cariostatic properties of fluoride.
Univ Estadual Campinas, Dept Physiol Sci, Fac Dent Piracicaba, Campinas, SP, Brazil
Univ Fed Alfenas, UNIFAL MG, Dept Pharm, Alfenas, MG, Brazil
State Univ Sao Paulo, Luiz de Queiroz Coll Agr, Dept Agrifood Ind Food & Nutr, Sao Paulo, SP, Brazil
Univ Rochester, Med Ctr, Ctr Oral Biol, Eastman Dept Dent, Rochester, NY 14627 USA
Univ Rochester, Med Ctr, Dept Microbiol & Immunol, Rochester, NY 14627 USA
Nat Prod Res Grp Oral Biol NatPROB, Sao Paulo, Brazil
State Univ Sao Paulo, Luiz de Queiroz Coll Agr, Dept Agrifood Ind Food & Nutr, Sao Paulo, SP, Brazil
FAPESP: 06/56379-4
CNPq: 304803/2005-7
USPHS Research grant from the National Institute of Dental and Craniofacial Research (National Institutes of Health, USA): R01 DE018023
CNPq: 141253/2005-3
CAPES: BEX 0154/06-7
