Author(s): Santos Bersot, Luciano dos ; Quintana Cavicchioli, Valeria ; Viana, Cibeli ; Konrad Burin, Raquel Cristina ; Camargo, Anderson Carlos ; Nogueira Pinto, Jose Paes de Almeida [UNESP] ; Nero, Luis Augusto ; Destro, Maria Teresa
Date: 2020
Persistent ID: http://hdl.handle.net/11449/196478
Origin: Oasisbr
Subject(s): Salmonella; pig production chain; PFGE; contamination routes; resistance
Made available in DSpace on 2020-12-10T19:46:17Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-12-01
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Control of Salmonella spp. in food production chains is very important to ensure safe foods and minimize the risks of foodborne disease occurrence. This study aimed to identify the prevalence and main contamination sources of Salmonella spp. in a pig production chain in southern Brazil. Six lots of piglets produced at different farms were tracked until their slaughter, and samples were subjected to Salmonella spp. detection. The obtained isolates were serotyped, subjected to antimicrobial resistance testing, and pulsed field gel electrophoresis (PFGE). Salmonella spp. was detected in 160 (10.2%) samples, and not detected in pig carcasses after final washing or chilling. Among the 210 Salmonella spp. isolates, S. Typhimurium was the most prevalent (n = 101) and resistant to at least one antimicrobial. High resistance rates were detected against tetracycline (83.8%), chloramphenicol (54.3%), and trimethoprim-sulfamethoxazole (33.3%). The isolates that were non-susceptible to three or more classes of antimicrobials (n = 60) were considered multidrug-resistant (MDR), and isolates resistant to up to six of the tested antimicrobials were found. PFGE allowed the identification of genetic diversity and demonstrated that farm environment and feed supply may be sources for the dissemination of Salmonella spp. along the production chain. The results revealed the sources of Salmonella contamination in the pig production chain and highlighted the risks of antimicrobial resistance spread.
Univ Fed Parana, Dept Ciencia Vet, Setor Palotina, BR-85950000 Palotina, Brazil
Univ Fed Vicosa, Dept Vet, BR-36570900 Vicosa, MG, Brazil
Univ Estadual Paulista, Dept Higiene Vet & Saude Publ, BR-18618000 Botucatu, SP, Brazil
Univ Sao Paulo, Fac Ciencias Farmaceut, BR-05508900 Sao Paulo, SP, Brazil
Univ Estadual Paulista, Dept Higiene Vet & Saude Publ, BR-18618000 Botucatu, SP, Brazil
