Author(s):
Bonfim, Caroline Measso do [UNESP] ; Monteleoni, Letícia Figueiredo [UNESP] ; Calmon, Marília de Freitas [UNESP] ; Cândido, Natália Maria [UNESP] ; Provazzi, Paola Jocelan Scarin [UNESP] ; Lino, Vanesca de Souza ; Rabachini, Tatiana ; Sichero, Laura ; Villa, Luisa Lina ; Quintana, Silvana Maria ; Melli, Patrícia Pereira dos Santos ; Primo, Fernando Lucas [UNESP] ; Amantino, Camila Fernanda ; Tedesco, Antonio Claudio ; Boccardo, Enrique ; Rahal, Paula [UNESP]
Date: 2020
Persistent ID: http://hdl.handle.net/11449/201554
Origin: Oasisbr
Subject(s): curcumin and photodynamic therapy; HPV; nanoemulsion; Vulvar intraepithelial neoplasia
Description
Made available in DSpace on 2020-12-12T02:35:41Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-01-01
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Vulvar intraepithelial neoplasia (VIN) is associated with human papillomavirus (HPV) infection. Curcumin is a natural bioactive compound with antineoplastic properties. The use of nanoparticles containing curcumin could allow a better performance of this compound in therapies. So, VIN biopsies were collected and HPV DNA detection was performed by PCR, positive samples were genotyped by Restriction Fragment Length Polymorphism (RFLP) and HPV-16 variants were determined by sequencing. HPV-16 positive vulva carcinoma cells (A431) were transduced with E-P and E-350G HPV-16 E6 variants. The viability of the transduced cells treated with nanoemulsions was determined by MTT assay. Besides, apoptosis was evaluated by enzymatic activity of Caspase-3/7. The cell viability assay showed that both the empty nanoemulsion (NE-V) and the nanoemulsion of curcumin (NE-CUR) had little effect on cell viability as compared to control cells. Additionally, we observed that cells irradiated in the presence of NE-CUR presented 90% of cell death. The apoptosis assay further revealed a significant increase in the activity of caspases 3 and 7 in A431 cells expressing both HPV-16 E6 variants after treatment with NE-CUR. Finally, we submitted the HPV transduced A431 cells to organotypic cultures and observed that the combination of treatments affected tissue architecture with evident signals of tissue damage. We concluded that nanoemulsions attain good biocompatibility, since no cytotoxicity was observed and NE-CUR associated with photoactivation showed promising results, leading to death only in cells subjected to irradiation. This drug delivery system associated with photodynamic therapy may become promising in the treatment of vulva lesions.
Laboratory of Genomic Studies Sao Paulo State University–UNESP
Department of Microbiology Institute of Biomedical Sciences University of São Paulo–USP
Institute of Pharmacology University of Bern
ICESP Center for Translational Research in Oncology- Instituto do Cancer do Estado de Sao Paulo
Departament of Radiology and Oncology School of Medicine Universidade de Sao Paulo–USP
Department of Gynecology and Obstetrics of the Ribeirão Preto Medical School USP Ribeirão Preto
Department of Bioprocess and Biotechnology Faculty of Pharmaceutical Sciences of Araraquara Universidade Estadual Paulista Julio de Mesquita Filho–UNESP Araraquara
Department of Chemistry Center for Nanotechnology and Tissue Engineering Faculty of Philosophy Sciences and Languages of Ribeirão Preto University of São Paulo–USP Ribeirão Preto
Laboratory of Genomic Studies Sao Paulo State University–UNESP
Department of Bioprocess and Biotechnology Faculty of Pharmaceutical Sciences of Araraquara Universidade Estadual Paulista Julio de Mesquita Filho–UNESP Araraquara
FAPESP: 2014/02064-9
