Author(s):
Rocha, Elerson Matos ; Marinotti, Osvaldo ; Serrão, Deidre Machado ; Correa, Laura Viana ; Katak, Ricardo de Melo ; Juan, Campos-de-Oliveir ; Muniz, Veranilce Alves ; de Oliveira, Marta Rodrigues ; Ferreira, Joaquim ; Pessoa, Marcos Cézar Fernandes ; Roque, Rosemary Aparecida ; da Mota, Adolfo Jose ; Onorati, Piero ; Souza-Neto, Jayme A. [UNESP] ; Terenius, Olle ; Tadei, Wanderli Pedro
Date: 2021
Persistent ID: http://hdl.handle.net/11449/205747
Origin: Oasisbr
Subject(s): Amazon forest; Malaria; Microbiota; Mosquito; Vector-borne disease
Description
Made available in DSpace on 2021-06-25T10:20:37Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-12-01
Uppsala Universitet
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Vetenskapsrådet
Background: Malaria remains a major public health problem in South America, mostly in the Amazon region. Among newly proposed ways of controlling malaria transmission to humans, paratransgenesis is a promising alternative. Paratransgenesis aims to inhibit the development of parasites within the vector through the action of genetically modified bacteria. The first step towards successful paratransgenesis in the Amazon is the identification of Anopheles darlingi symbiotic bacteria, which are transmitted vertically among mosquitoes, and are not pathogenic to humans. Methods: Culturable bacteria associated with An. darlingi and their breeding sites were isolated by conventional microbiological techniques. Isolated strains were transformed with a GFP expressing plasmid, pSPT-1-GFP, and reintroduced in mosquitoes by feeding. Their survival and persistence in the next generation was assessed by the isolation of fluorescent bacteria from eggs, larvae, pupae and adult homogenates. Results: A total of 179 bacterial strains were isolated from samples from two locations, Coari and Manaus. The predominant genera identified in this study were Acinetobacter, Enterobacter, Klebsiella, Serratia, Bacillus, Elizabethkingia, Stenotrophomonas and Pantoea. Two isolated strains, Serratia-Adu40 and Pantoea-Ovo3, were successfully transformed with the pSPT-1-GFP plasmid and expressed GFP. The fluorescent bacteria fed to adult females were transferred to their eggs, which persisted in larvae and throughout metamorphosis, and were detected in adult mosquitoes of the next generation. Conclusion: Serratia-Adu40 and Pantoea-Ovo3 are promising candidates for paratransgenesis in An. darlingi. Further research is needed to determine if these bacteria are vertically transferred in nature.
Programa de Pós-Graduação em Biotecnologia Universidade Federal do Amazonas - PPGBIOTEC / UFAM
MTEKPrime
Universidade Estadual do Amazonas - MBT UEA
Universidade Estadual do Amazonas - BIONORTE UEA
Laboratório de Malária E Dengue Instituto Nacional de Pesquisas da Amazônia INPA
Department of Ecology Swedish University of Agricultural Sciences (SLU), Box 7044
School of Agricultural Sciences Department of Bioprocesses and Biotechnology Central Multi User Laboratory São Paulo State University (UNESP)
Department of Cell and Molecular Biology Microbiology Uppsala University, Box 596
School of Agricultural Sciences Department of Bioprocesses and Biotechnology Central Multi User Laboratory São Paulo State University (UNESP)
CAPES: 001
Vetenskapsrådet: 348-2012-622
