Autor(es):
Carneiro, Victor Alves ; de Oliveira, Simone Torres ; Silva, Rondinely Lima ; Duarte, Humberlania de Sousa ; Silva, Maria Laína ; Matos, Maria Nágila Carneiro ; Cavalcante, Rafaela Mesquita Bastos ; Figueira, Ciro Siqueira ; Lorenzón, Esteban Nicolás ; Cilli, Eduardo Maffud [UNESP] ; da Cunha, Rodrigo Maranguape Silva
Data: 2021
Identificador Persistente: http://hdl.handle.net/11449/207966
Origem: Oasisbr
Assunto(s): Antibiofilm activity; Antimicrobial peptide; Ciprofloxacin; Lys-a1; Pseudomonas aeruginosa; Synergism
Descrição
Made available in DSpace on 2021-06-25T11:04:10Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-01-01
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Rijksuniversiteit Groningen
Background: Ciprofloxacin (Cip) is the most commonly used quinolone in clinical prac-tice; however large-scale use has favored the increase of multiresistant pathogenic microorganisms. Antimicrobial peptides (AMPs) appear to be a promising alternative in potentiating these conven-tional drugs. Objective: The aim of this study was to evaluate the effect of the peptide Lys-[Trp6]hy-a1 (lys-a1) on the antimicrobial and antibiofilm activity of ciprofloxacin against clinically relevant gram-nega-tive bacteria. Methods: The antimicrobial effects of Cip and lys-a1 were assessed by determining the minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs). The synergistic action of Cip and lys-a1 was determined by checkerboard assay. The time-kill curve was con-structed for the Cip/lys-a1 combination against Pseudomonas aeruginosa ATCC 9027. The antibio-film activity of this combination was analyzed by crystal violet, colony-forming unit count and atomic force microscopy (AFM). Results: The data demonstrated that lys-a1 was able to inhibit planktonic growth of strains of P. aeruginosa and Klebsiella pneumoniae both at 125 μg/mL. The fractional inhibitory concentration index (FICi) showed a synergistic effect between Cip and lys-a1 against P. aeruginosa, decreasing the MICs of the individual antimicrobial agents by 4-and 8-fold, respectively. This effect was also observed for the death kinetics and antibiofilm activity. Analysis of the early biofilms (6 h) as well as isolated cells by AFM images evidenced the cell perturbation caused by Cip/lys-a1 treatment. Conclusion: These data suggest that lys-a1 has biotechnological potential as a therapeutic tool for the treatment of infections caused by clinically relevant microorganisms, especially P. aeruginosa.
Laboratory of Biofilms and Antimicrobial Agents (LaBAM) Faculty of Medicine Federal University of Ceara – UFC
Center for Bioprospecting and Applied Molecular Experimentation (NUBEM) University Center IN-TA – UNINTA
Medical Research Laboratory Federal University of Jatai
Department of Biochemistry and Chemical Technology Estadual University of Sao Paulo – UNESP
Department of Biochemistry and Chemical Technology Estadual University of Sao Paulo – UNESP
