Author(s): Salles, Tais Helena Costa ; Volpe-Zanutto, Fabiana ; De Oliveira Sousa, Ilza Maria ; MacHado, Daisy ; Zanatta, Ana Caroline [UNESP] ; Vilegas, Wagner [UNESP] ; Lancellotti, Marcelo ; Foglio, Mary Ann ; D'Avila, Marcos Akira
Date: 2021
Persistent ID: http://hdl.handle.net/11449/207982
Origin: Oasisbr
Subject(s): Arrabidaea chica; Guided tissue regeneration; Poly (ϵ-caprolactone); Pterodon pubescens
Made available in DSpace on 2021-06-25T11:04:22Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-11-01
The guided tissue regeneration (GTR) technique can be applied in dentistry and other medical specializations, such as orthopedics. In modern dentistry, GTR has been used in periodontics and implantology to treat periodontal defects, to reconstruct lost, damaged and atrophied bone tissue in dental implant procedures, and to preserve alveolar bases after tooth extraction. In order to create and improve new therapies and to develop new biomaterials that restore, improve and prevent aggravation of compromised tissue function, poly (ϵ-caprolactone) (PCL) polymer membranes were obtained by the electrospinning process and were associated with two plant extracts: Pterodon pubescens Benth (P. pubescens) and Arrabidaea chica Verlot (A. chica) which are characterized by their pharmacological activities of anti-inflammatory and healing actions, respectively. Fiber morphology was analyzed using scanning electron microscopy (SEM), where fiber average diameter was measured from SEM images. Contact angle measurements were performed in order to evaluate the hydrophilicity of electrospun membranes containing vegetal extract. High-performance liquid chromatography was used to evaluate the ability to release active ingredients. Cytotoxicity and cell proliferation assays were performed in vitro on NIH-3T3 cells for 1, 3 and 7 d. Electrospun PCL membranes associated with plant extracts P. pubescens and/or A. chica presented a controlled release profile of the active compounds induced fibroblast formation, suggesting that they are promising and suitable for applications in GTR.
Department of Manufacturing and Materials Engineering School of Mechanical Engineering University of Campinas UNICAMP
School of Pharmacy Queen's University Belfast
Graduate School of Bioscience and Technology of Bioactive Products Biology Institute University at Campinas
Faculdade de Ciências Médicas Universidade Estadual de Campinas UNICAMP
Faculty of Pharmaceutical Science University at Campinas
Instituto de Biociencias Univ Estadual Paulista UNESP
Instituto de Biociencias Univ Estadual Paulista UNESP
