Author(s): Pinto, Meri Emili F. [UNESP] ; Chan, Lai Yue ; Koehbach, Johannes ; Devi, Seema ; Gründemann, Carsten ; Gruber, Christian W. ; Gomes, Mario ; Bolzani, Vanderlan S. [UNESP] ; Cilli, Eduardo Maffud [UNESP] ; Craik, David J.
Date: 2021
Persistent ID: http://hdl.handle.net/11449/208356
Origin: Oasisbr
Made available in DSpace on 2021-06-25T11:10:52Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-22
Cyclotides are plant-derived peptides found within five families of flowering plants (Violaceae, Rubiaceae, Fabaceae, Solanaceae, and Poaceae) that have a cyclic backbone and six conserved cysteine residues linked by disulfide bonds. Their presence within the Violaceae species seems ubiquitous, yet not all members of other families produce these macrocyclic peptides. The genus Palicourea Aubl. (Rubiaceae) contains hundreds of neotropical species of shrubs and small trees; however, only a few cyclotides have been discovered hitherto. Herein, five previously uncharacterized Möbius cyclotides within Palicourea sessilis and their pharmacological activities are described. Cyclotides were isolated from leaves and stems of this plant and identified as pase A-E, as well as the known peptide kalata S. Cyclotides were de novo sequenced by MALDI-TOF/TOF mass spectrometry, and their structures were solved by NMR spectroscopy. Because some cyclotides have been reported to modulate immune cells, pase A-D were assayed for cell proliferation of human primary activated T lymphocytes, and the results showed a dose-dependent antiproliferative function. The toxicity on other nonimmune cells was also assessed. This study reveals that pase cyclotides have potential for applications as immunosuppressants and in immune-related disorders.
Institute of Chemistry Saõ Paulo State University UNESP
Institute for Molecular Bioscience Australian Research Council Centre of Excellence for Innovations in Peptide and Protein Science University of Queensland
Institute for Infection Prevention and Hospital Epidemiology Center for Complementary Medicine University of Freiburg
Translational Complementary Medicine Department of Pharmaceutical Sciences University of Basel
Center for Physiology and Pharmacology Medical University of Vienna
Rio de Janeiro Botanic Garden Research Institute-JBRJ
Institute of Chemistry Saõ Paulo State University UNESP
