Author(s): Costa-Fernandez, Sandra ; Matos, Jenyffer K.R. ; Scheunemann, Gaby S. ; Salata, Giovanna C. ; Chorilli, Marlus [UNESP] ; Watanabe, Il-Sei ; de Araujo, Gabriel L.B. ; Santos, Marinilce F. ; Ishida, Kelly ; Lopes, Luciana B.
Date: 2021
Persistent ID: http://hdl.handle.net/11449/208652
Origin: Oasisbr
Subject(s): Antimicrobial; Antioxidants; Bioadhesion; Nanostructured lipid carriers; Wound healing
Made available in DSpace on 2021-06-25T11:15:38Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-07-31
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
The high incidence and costs of chronic wounds in the elderly have motivated the search for innovations to improve product performance and the healing process while reducing costs. In this study, bioadhesive nanostructured lipid carriers (NLC) were developed for the co-encapsulation of compounds with antioxidant (α-tocopherol and quercetin) and antimicrobial (tea tree oil) activity for management of wounds. The NLC was produced with shea butter and argan oil, and modified with sodium alginate or chitosan to confer bioadhesive properties. Spherical nanoparticles of ~307–330 nm and zeta potential varying from −21.2 to +11.8 mV were obtained. Thermal analysis demonstrated that the lipid matrix reduced tea tree oil thermal loss (~1.8-fold). Regardless of the type of polysaccharide employed, the NLCs promoted cutaneous localization of antioxidants in damaged (subjected to incision) skin, with a ~74 to 180-fold higher delivery into the skin compared to percutaneous delivery. This result is consistent with the similar bioadhesive properties of chitosan or sodium alginate-modified NLC. Nanoencapsulation of tea tree oil did not preclude its antimicrobial effects against susceptible and resistant strains of S. aureus and P. aeruginosa, while co-encapsulation of antioxidants increased the NLC-induced fibroblasts migration, supporting their potential usefulness for management of wounds.
Instituto de Ciências Biomédicas Universidade de São Paulo
School of Pharmaceutical Sciences São Paulo State University (UNESP)
Faculdade de Ciências Farmacêuticas Universidade de São Paulo
School of Pharmaceutical Sciences São Paulo State University (UNESP)
CAPES: 001
FAPESP: 2013/16617-7
FAPESP: 2015/07993-0
FAPESP: 2017/19059-6
FAPESP: 2017/19374-9
FAPESP: 2018/13877-1
FAPESP: 2019/26048-6
CNPq: 306866/2020-0
