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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Instituto Federal Goiano
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Fundacao de Amaparo a Pesquisa do Estado de Goias (FAPEG, Brazil)
Artemisinin extracted from Artemisia annua L. plants has a range of properties that qualifies it to treat several diseases, such as malaria and cancer. However, it has short half-life, which requires making continuous use of it, which has motivated the association of artemisinin (ART) with polymeric nanoparticles to increase its therapeutic efficiency. However, the ecotoxicological safety of this association has been questioned, given the scarcity of studies in this area. Thus, in this work the toxicity of Poly (epsilon-Caprolactone) nanocapsules added with ART (ART-NANO) in zebrafish (Danio rerio), embryos and adults was studied. Different endpoints were analyzed in organisms exposed to ART-NANO, including those predictive of embryotoxicity and histopatoxicity. Embryotoxicity was analyzed based on Organization for Economic Co-operation and Development (OECD) test guideline (236) for fish embryo acute toxicity applied to zebrafish (Danio rerio) at 96 hpf under five nominal logarithmic concentrations (0.125 to 2.0 mg/L). Our results demonstrate, mainly, that fertilized eggs presented increased coagulation, lack of heart rate, vitelline sac displacement and lack of somite formation. On the other hand, adult individuals (exposed to the same concentrations and evaluated after 24 and 96 h of exposure) have shown increased pericarditis. Therefore, the treatment based on ART, poly (epsilon-caprolactone) nanocapsules and on their combination at different concentrations have shown toxic effects on zebrafish embryos and adult individuals. (C) 2020 Elsevier B.V. All rights reserved.
Univ Sao Paulo, Biomed Sci Inst, Pharmacol Dept, Sao Paulo, SP, Brazil
Fed Univ Rio Janeiro UFRJ, Biodivers & Sustainabil Inst NUPEM, Integrated Translat Biosci Lab LIBT, Macae, RJ, Brazil
Univ Fed Sao Carlos, Dept Genet & Evolut, Sao Carlos, SP, Brazil
Univ Estadual Campinas, Pharmaceut Sci Sch, Campinas, SP, Brazil
Univ Fed Minas Gerais, Belo Horizonte, MG, Brazil
Sao Paulo State Univ, Jaboticabal, SP, Brazil
Univ Fed Alfenas, Motric Sci Inst, Alfenas, MG, Brazil
Livestock & Supply, Minist Agr, Sao Carlos, SP, Brazil
Sao Paulo State Univ, Botucatu, SP, Brazil
Goiano Fed Inst, Biol Sci Dept, Urutai, Go, Brazil
Sao Paulo State Univ, Jaboticabal, SP, Brazil
Sao Paulo State Univ, Botucatu, SP, Brazil
CNPq: 426531/2018-3
Instituto Federal Goiano: 23219.000865.2020-78
CNPq: 307743/2018-7
