Author(s): Alvarez, Marcus Vinicius Niz [UNESP] ; Alonso, Diego Peres [UNESP] ; Kadri, Samir Moura [UNESP] ; Rufalco-Moutinho, Paulo ; Bernardes, Isabella Ariadne Ferrari [UNESP] ; de Mello, Ana Carolina Florindo [UNESP] ; Souto, Ana Carolina [UNESP] ; Carrasco-Escobar, Gabriel ; Moreno, Marta ; Gamboa, Dionicia ; Vinetz, Joseph M. ; Conn, Jan E. ; Ribolla, Paulo E. M. [UNESP]
Date: 2022
Persistent ID: http://hdl.handle.net/11449/223726
Origin: Oasisbr
Made available in DSpace on 2022-04-28T19:52:46Z (GMT). No. of bitstreams: 0 Previous issue date: 2022-12-01
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Fogarty International Center
Background: In Brazil, malaria is concentrated in the Amazon Basin, where more than 99% of the annual cases are reported. The main goal of this study was to investigate the population structure and genetic association of the biting behavior of Nyssorhynchus (also known as Anopheles) darlingi, the major malaria vector in the Amazon region of Brazil, using low-coverage genomic sequencing data. Methods: Samples were collected in the municipality of Mâncio Lima, Acre state, Brazil between 2016 and 2017. Different approaches using genotype imputation and no gene imputation for data treatment and low-coverage sequencing genotyping were performed. After the samples were genotyped, population stratification analysis was performed. Results: Weak but statistically significant stratification signatures were identified between subpopulations separated by distances of approximately 2–3 km. Genome-wide association studies (GWAS) were performed to compare indoor/outdoor biting behavior and blood-seeking at dusk/dawn. A statistically significant association was observed between biting behavior and single nucleotide polymorphism (SNP) markers adjacent to the gene associated with cytochrome P450 (CYP) 4H14, which is associated with insecticide resistance. A statistically significant association between blood-seeking periodicity and SNP markers adjacent to genes associated with the circadian cycle was also observed. Conclusion: The data presented here suggest that low-coverage whole-genome sequencing with adequate processing is a powerful tool to genetically characterize vector populations at a microgeographic scale in malaria transmission areas, as well as for use in GWAS. Female mosquitoes entering houses to take a blood meal may be related to a specific CYP4H14 allele, and female timing of blood-seeking is related to circadian rhythm genes. Graphical Abstract: [Figure not available: see fulltext.]
Sao Paulo State University (UNESP)
Nucleo de Medicina Tropical Universidade de Brasília
Laboratorio ICEMR-Amazonia Laboratorios de Investigación Y Desarrollo Facultad de Ciencias Y Filosofia Universidad Peruana Cayetano Heredia
Facultad de Salud Pública Universidad Peruana Cayetano Heredia
Department of Immunology and Infection London School of Hygiene and Tropical Medicine
Departamento de Ciencias Celulares Y Moleculares Facultad de Ciencias Y Filosofía Universidad Peruana Cayetano Heredia
Instituto de Medicina Tropical Alexander Von Humboldt Universidad Peruana Cayetano Heredia
Section of Infectious Diseases Department of Internal Medicine Yale School of Medicine
Wadsworth Center New York State Department of Health
Department of Biomedical Sciences School of Public Health State University of New York at Albany
Sao Paulo State University (UNESP)
FAPESP: 2018/07406-6
Fogarty International Center: D43 TW007120
