Autor(es): Da Silva, Marcelo Aparecido [UNESP] ; Cardoso, Claudia Andréa Lima ; Vilegas, Wagner [UNESP] ; Dos Santos, Lourdes Campaner [UNESP]
Data: 2022
Identificador Persistente: http://hdl.handle.net/11449/225723
Origem: Oasisbr
Assunto(s): Eriocaulaceae; Flavonoids; Liquid chromatography; Quantification
Made available in DSpace on 2022-04-28T20:59:05Z (GMT). No. of bitstreams: 0 Previous issue date: 2009-11-01
Quantification of prepared samples by analysis using high performance liquid chromatography with DAD detection was developed to analyze rutin, 6-methoxyapigenin, and 6-methoxyapigenin-7-O-β-D-glucopyranoside isolated from methanolic extracts of different genus: Syngonanthus, Leiothix and Eriocaulon (Eriocaulaceae). The linearity, accuracy, and the inter-day precision of the procedure were evaluated. The calibration curves were linear. The recoveries of the flavonoids in the samples analyzed were 96.3% to 98.5%. The percentage coefficient of variation for the quantitative analysis of the flavonoids in the analyses of the samples was under 5%. The antimicrobial activity of the five methanol extracts of these Eriocaulaceae species was assayed against the microorganisms Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Salmonella setubal, Saccharomyces cerevisiae and Candida albicans. Measured MIC values ranged from 1.25 to 10.00 mg/mL. The flavonoid contents suggest that Eriocaulaceae species may be a promising source of compounds to produce natural phytomedicines.
UNESP-Univ Estadual Paulista Instituto de Química Departamento de Química Orgânica, CP 355, CEP 14800-900, Araraquara, São Paulo
UEMS Course of Chemistry Mato Grosso do Sul State University, CP 351, CEP 79804-970, Dourados, Mato Grosso do Sul
UNESP-Univ Estadual Paulista Instituto de Química Departamento de Química Orgânica, CP 355, CEP 14800-900, Araraquara, São Paulo
