Author(s): Manechini, João Ricardo Vieira ; Santos, Paulo Henrique da Silva [UNESP] ; Romanel, Elisson ; Brito, Michael dos Santos ; Scarpari, Maximiliano Salles ; Jackson, Stephen ; Pinto, Luciana Rossini [UNESP] ; Vicentini, Renato
Date: 2022
Persistent ID: http://hdl.handle.net/11449/229071
Origin: Oasisbr
Subject(s): artificial induction; flowering; photoperiodism; sugarcane; transcriptome
Made available in DSpace on 2022-04-29T08:30:14Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-06-15
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Flowering is of utmost relevance for the agricultural productivity of the sugarcane bioeconomy, but data and knowledge of the genetic mechanisms underlying its photoperiodic induction are still scarce. An understanding of the molecular mechanisms that regulate the transition from vegetative to reproductive growth in sugarcane could provide better control of flowering for breeding. This study aimed to investigate the transcriptome of +1 mature leaves of a sugarcane cultivar subjected to florally inductive and non-inductive photoperiodic treatments to identify gene expression patterns and molecular regulatory modules. We identified 7,083 differentially expressed (DE) genes, of which 5,623 showed significant identity to other plant genes. Functional group analysis showed differential regulation of important metabolic pathways involved in plant development, such as plant hormones (i.e., cytokinin, gibberellin, and abscisic acid), light reactions, and photorespiration. Gene ontology enrichment analysis revealed evidence of upregulated processes and functions related to the response to abiotic stress, photoprotection, photosynthesis, light harvesting, and pigment biosynthesis, whereas important categories related to growth and vegetative development of plants, such as plant organ morphogenesis, shoot system development, macromolecule metabolic process, and lignin biosynthesis, were downregulated. Also, out of 76 sugarcane transcripts considered putative orthologs to flowering genes from other plants (such as Arabidopsis thaliana, Oryza sativa, and Sorghum bicolor), 21 transcripts were DE. Nine DE genes related to flowering and response to photoperiod were analyzed either at mature or spindle leaves at two development stages corresponding to the early stage of induction and inflorescence primordia formation. Finally, we report a set of flowering-induced long non-coding RNAs and describe their level of conservation to other crops, many of which showed expression patterns correlated against those in the functionally grouped gene network.
Laboratório de Biologia de Sistemas Departamento de Genética Evolução Microbiologia e Imunologia Instituto de Biologia Universidade Estadual de Campinas (UNICAMP)
Departamento de Genética e Melhoramento de Plantas Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual de São Paulo (UNESP)
Laboratório de Genômica de Plantas e Bioenergia (PGEMBL) Departamento de Biotecnologia Escola de Engenharia de Lorena (EEL) Universidade de São Paulo (USP)
Instituto de Ciência e Tecnologia Universidade Federal de São Paulo (UNIFESP)
Centro de Cana Instituto Agronômico de Campinas (IAC)
School of Life Sciences The University of Warwick
Departamento de Genética e Melhoramento de Plantas Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual de São Paulo (UNESP)
